Genome location: Pf3D7_13_v3:2,619,004..2,621,127(+)
Genome classification: Core
Product Description: aminomethyltransferase, mitochondrial, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.2.10 (Aminomethyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Function | GO:0004047 | aminomethyltransferase activity |
| Process | GO:0006546 | glycine catabolic process |
| Process | GO:0019464 | glycine decarboxylation via glycine cleavage system |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.18 | 0.91 |
| Trophozoite | expressed | 0.80 | 0.56 |
| Schizont | expressed | 0.13 | 0.92 |
| Gametocyte | expressed | 0.52 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1365500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.468 | MFS: -2.673 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1141300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1141300)
Modification: Disrupted | RMgm-3140
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6T0
"Best" AlphaFill ligand hit: C2F (5-methyl-5,6,7,8-tetrahydrofolic acid, Local RMSD=0.10) with 3A8I (Global RMSD=2.35)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.2.10 | aminomethyltransferase | iodoacetamideN-ethylmaleimide |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101103
Most Similar Human Ortholog: B3KTU4
TM-align score: 0.97 | RMSD: 1.05
Seq Identity: 0.35 | Length: 351 / 406
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000145020 | aminomethyltransferase |
| ENSG00000283189 | novel protein |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 31 | 9 | 23 |
| disruptive | 4 | 18 | 16 | 40 |
| missense | 4 | 18 | 16 | 36 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 46 | 10 | 29 |
| disruptive | 6 | 59 | 37 | 58 |
| missense | 5 | 50 | 36 | 35 |
PlasmoDB Total SNPs: 162
Non-coding: 131 | Synonymous: 24 | Nonsynonymous: 6 | Stop Codon: 1
Protein Length: 406 | Molecular Weight (kDa): 46.903
UniProt ID(s): Q8I6T0
PDB ID(s): None
Isoelectric Point: 8.79
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006222 | Aminomethyltransferase, folate-binding domain |
| InterPro | IPR013977 | Glycine cleavage T-protein, C-terminal barrel domain |
| InterPro | IPR028896 | Aminomethyltransferase-like |
| InterPro | IPR029043 | Glycine cleavage T-protein/YgfZ, C-terminal |
| PFam | PF01571 | Aminomethyltransferase, folate-binding domain |
| PFam | PF08669 | Glycine cleavage T-protein, C-terminal barrel domain |
| Superfamily | SSF101790 | Glycine cleavage T-protein/YgfZ, C-terminal |
| Superfamily | SSF103025 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25454081 | Plasmodium berghei glycine cleavage system T-protein is non-essential forparasite survival in vertebrate and invertebrate hosts. | Varadarajan NM, Sundaram B, ..., Nagaraj VA | 10.1016/j.molbiopara.2014.10.003 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |