Genome location: Pf3D7_13_v3:2,621,160..2,624,082(+)
Genome classification: Core
Product Description: DNA topoisomerase 6 subunit B
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0015935 | small ribosomal subunit |
| Function | GO:0003916 | DNA topoisomerase activity |
| Function | GO:0003824 | catalytic activity |
| Function | GO:0042802 | identical protein binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.91 |
| Trophozoite | expressed | 0.26 | 0.84 |
| Schizont | expressed | 0.63 | 0.63 |
| Gametocyte | expressed | 0.57 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1365600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.062 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1141400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1141400)
Modification: Disrupted | RMgm-3141
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID53
"Best" AlphaFill ligand hit: RDC (monorden, Local RMSD=1.55) with 2HKJ (Global RMSD=10.85)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 35 | 20 | 42 |
| disruptive | 22 | 99 | 62 | 115 |
| missense | 15 | 84 | 58 | 104 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 64 | 34 | 49 |
| disruptive | 43 | 183 | 89 | 128 |
| missense | 26 | 166 | 81 | 94 |
PlasmoDB Total SNPs: 203
Non-coding: 141 | Synonymous: 39 | Nonsynonymous: 22 | Stop Codon: 1
Protein Length: 561 | Molecular Weight (kDa): 66.083
UniProt ID(s): Q8ID53
PDB ID(s): None
Isoelectric Point: 4.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003594 | Histidine kinase/HSP90-like ATPase |
| InterPro | IPR036890 | Histidine kinase/HSP90-like ATPase superfamily |
| PFam | PF02518 | Histidine kinase/HSP90-like ATPase |
| Superfamily | SSF55874 | Histidine kinase/HSP90-like ATPase superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 27303712 | Radicicol-Mediated Inhibition of Topoisomerase V IB-VIA Activity of the Human Malaria Parasite Plasmodium falciparum | Chalapareddy S, Chakrabarty S, Bhattacharyya MK, Bhattacharyya S | 10.1128/mSphere.00025-15 |