PF3D7_1366400 (RHOP148)

Genome location: Pf3D7_13_v3:2,654,471..2,660,689(-)

Genome classification: Core

Function and Localization

Product Description: rhoptry protein RHOP148

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020008	rhoptry

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.38	0.82
Trophozoite	expressed	1.07	0.46
Schizont	expressed	0.37	0.78
Gametocyte	expressed	0.54	0.69

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1366400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.133 | MFS: -2.943 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1142300)

Modification: Disrupted | RMgm-3144

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K9G4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533208

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	18	89	46	92
disruptive	92	246	121	224
missense	42	169	90	166

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	35	177	56	93
disruptive	159	434	162	401
missense	73	316	117	242

PlasmoDB Total SNPs: 453

Non-coding: 191 | Synonymous: 137 | Nonsynonymous: 125 | Stop Codon: 0

Protein Information

Protein Length: 1262 | Molecular Weight (kDa): 148.755

UniProt ID(s): A0A5K1K9G4, C5HEX8, C5HEY0, C5HEY1, C5HEY2, C5HEY3, C5HEY4, C5HEY5, C5HEY6, C5HEZ2, Q868L5

PDB ID(s): None

Isoelectric Point: 8.72

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12706797	Characterization of PfRhop148, a novel rhoptry protein of Plasmodium falciparum.	Lobo CA, Rodriguez M, ..., Lustigman S	10.1016/s0166-6851(03)00050-1
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104