PF3D7_1367500

Genome location: Pf3D7_13_v3:2,691,361..2,693,825(+)

Genome classification: Core

Function and Localization

Product Description: NADH-cytochrome b5 reductase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 1.6.2.2 (Cytochrome-b5 reductase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020020	food vacuole
Function	GO:0071949	FAD binding
Function	GO:0009055	electron transfer activity
Process	GO:0055114	oxidation-reduction process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.10	0.95
Trophozoite	expressed	0.53	0.69
Schizont	expressed	0.33	0.79
Gametocyte	expressed	0.39	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1367500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.187 | MFS: -3.347 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1143400)

Relative Growth Rate: 0.92 ± 0.12
Confidence: 5.65

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1143400)

Modification: Disrupted | RMgm-4619

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ID33

"Best" AlphaFill ligand hit: FAD (flavin-adenine dinucleotide, Local RMSD=0.25) with 2EIX (Global RMSD=3.43)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.6.2.2	cytochrome-b5 reductase	ADPNAD+NADP+MgCl2CaCl2heminrutinmorinAmytalacetatecitrateebselen...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100179

Most Similar Human Ortholog: Q6BCY4

TM-align score: 0.88 | RMSD: 2.00

Seq Identity: 0.28 | Length: 260 / 362

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100243	cytochrome b5 reductase 3
ENSG00000139531	sulfite oxidase
ENSG00000159348	cytochrome b5 reductase 1
ENSG00000166394	cytochrome b5 reductase 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	25	16	29
disruptive	10	25	15	31
missense	10	25	15	29

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	56	13	29
disruptive	18	50	32	80
missense	18	43	27	53

PlasmoDB Total SNPs: 132

Non-coding: 116 | Synonymous: 8 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 362 | Molecular Weight (kDa): 42.596

UniProt ID(s): Q8ID33

PDB ID(s): None

Isoelectric Point: 9.66

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001433	Oxidoreductase FAD/NAD(P)-binding
InterPro	IPR001834	NADH:cytochrome b5 reductase-like
InterPro	IPR008333	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain
InterPro	IPR017938	Riboflavin synthase-like beta-barrel
InterPro	IPR039261	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
PFam	PF00175	Oxidoreductase FAD/NAD(P)-binding
PFam	PF00970	Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domain
Superfamily	SSF52343	Ferredoxin-NADP reductase (FNR), nucleotide-binding domain
Superfamily	SSF63380	Riboflavin synthase-like beta-barrel