Genome location: Pf3D7_13_v3:2,695,379..2,700,285(+)
Genome classification: Core
Product Description: alanine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.7 (Alanine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004813 | alanine-tRNA ligase activity |
| Function | GO:0016597 | amino acid binding |
| Function | GO:0002161 | aminoacyl-tRNA editing activity |
| Process | GO:0006419 | alanyl-tRNA aminoacylation |
| Process | GO:0006400 | tRNA modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.40 | 0.42 |
| Trophozoite | expressed | 1.64 | 0.26 |
| Schizont | expressed | 0.34 | 0.81 |
| Gametocyte | expressed | 0.72 | 0.62 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1367700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.127 | MFS: -2.921 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1143600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID31
"Best" AlphaFill ligand hit: HED (2-hydroxyethyl disulfide, Local RMSD=0.26) with 3HXV (Global RMSD=3.85)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.7 | alanine-tRNA ligase | NEML-cysteineL-glutathionemonobromobimane2-mercaptoethanol2-mercaptoethylamineelongation factor Tuguanidine hydrochloridep-Substituted mercuribenzoate5,5'-dithiobis(2-nitrobenzoate)p-Hydroxymercuriphenylsulfonic acid5'-O-[N-(L-alanyl)-sulfamoyl]adenosine... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100414
Most Similar Human Ortholog: A0A6Q8PG62
TM-align score: 0.98 | RMSD: 0.94
Seq Identity: 0.60 | Length: 277 / 1408
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000090861 | alanyl-tRNA synthetase 1 |
| ENSG00000124608 | alanyl-tRNA synthetase 2, mitochondrial |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 96 | 37 | 88 |
| disruptive | 42 | 236 | 123 | 276 |
| missense | 27 | 202 | 111 | 226 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 34 | 159 | 52 | 85 |
| disruptive | 89 | 460 | 200 | 356 |
| missense | 61 | 360 | 172 | 241 |
PlasmoDB Total SNPs: 179
Non-coding: 40 | Synonymous: 61 | Nonsynonymous: 78 | Stop Codon: 0
Protein Length: 1408 | Molecular Weight (kDa): 165.135
UniProt ID(s): Q8ID31
PDB ID(s): None
Isoelectric Point: 9.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002318 | Alanine-tRNA ligase, class IIc |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR012947 | Threonyl/alanyl tRNA synthetase, SAD |
| InterPro | IPR018162 | Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily |
| InterPro | IPR018163 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily |
| InterPro | IPR018164 | Alanyl-tRNA synthetase, class IIc, N-terminal |
| InterPro | IPR023033 | Alanine-tRNA ligase, eukaryota/bacteria |
| PFam | PF01411 | Alanyl-tRNA synthetase, class IIc, N-terminal |
| PFam | PF07973 | Threonyl/alanyl tRNA synthetase, SAD |
| Superfamily | SSF101353 | Alanine-tRNA ligase, class IIc, anti-codon-binding domain superfamily |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF55186 | Threonyl/alanyl tRNA synthetase, class II-like, putative editing domain superfamily |
| Superfamily | SSF55681 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22222968 | Dual targeting of aminoacyl-tRNA synthetases to the apicoplast and cytosol inPlasmodium falciparum. | Jackson KE, Pham JS, ..., Ralph SA | 10.1016/j.ijpara.2011.11.008 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 34500470 | Impact of alanyl-tRNA synthetase editing deficiency in yeast | Zhang H, Wu J, Lyu Z, Ling J | 10.1093/nar/gkab766 |