Genome location: Pf3D7_13_v3:2,709,995..2,711,880(-)
Genome classification: Core
Product Description: 26S proteasome regulatory subunit RPN11, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0005838 | proteasome regulatory particle |
| Component | GO:0008541 | proteasome regulatory particle, lid subcomplex |
| Function | GO:0008237 | metallopeptidase activity |
| Function | GO:0070628 | proteasome binding |
| Function | GO:0004843 | thiol-dependent deubiquitinase |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0016579 | protein deubiquitination |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.42 | 0.74 |
| Schizont | expressed | 0.36 | 0.76 |
| Gametocyte | expressed | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1368100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.138 | MFS: -3.032 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ID28
"Best" AlphaFill ligand hit: ETE (2-{2-[2-2-(methoxy-ethoxy)-ethoxy]-ethoxy}-ethanol, Local RMSD=2.37) with 2O95 (Global RMSD=3.18)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O00487 | 26S proteasome non-ATPase regulatory subunit 14 | HOGENOM, OMA, OrthoDB | MLS000056404MLS000046765cid_2269367MLS000549030... | |
| Q92905 | COP9 signalosome complex subunit 5 | OrthoDB | US10005735, Compound 7US10005735, Compound 10US10005735, Compound 11US10005735, Compound 15... |
Ortholog Group (OrthoMCL): OG6_101835
Most Similar Human Ortholog: O00487
TM-align score: 0.90 | RMSD: 1.46
Seq Identity: 0.62 | Length: 290 / 311
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000115233 | proteasome 26S subunit, non-ATPase 14 |
Protein Length: 311 | Molecular Weight (kDa): 35.212
UniProt ID(s): Q8ID28
PDB ID(s): None
Isoelectric Point: 6.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000555 | JAB1/MPN/MOV34 metalloenzyme domain |
| InterPro | IPR024969 | Rpn11/EIF3F, C-terminal |
| InterPro | IPR035299 | 26S Proteasome non-ATPase regulatory subunit 14 |
| PFam | PF01398 | JAB1/MPN/MOV34 metalloenzyme domain |
| PFam | PF13012 | Rpn11/EIF3F, C-terminal |
| Superfamily | SSF102712 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 22 | 6 | 12 |
| disruptive | 2 | 1 | 1 | 8 |
| missense | 2 | 1 | 1 | 5 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 33 | 8 | 13 |
| disruptive | 6 | 11 | 12 | 59 |
| missense | 2 | 6 | 6 | 39 |
PlasmoDB Total SNPs: 149
Non-coding: 137 | Synonymous: 11 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 36241058 | Novel insights into the non-canonical roles of PSMD14/POH1/Rpn11 in proteostasis and in the modulation of cancer progression | Bustamante HA, Albornoz N, ..., Burgos PV | 10.1016/j.cellsig.2022.110490 |
| 35501388 | POH1/Rpn11/PSMD14: a journey from basic research in fission yeast to a prognostic marker and a druggable target in cancer cells | Spataro V, Buetti-Dinh A | 10.1038/s41416-022-01829-z |
| 34627761 | Activity of the Giardia intestinalis proteasome during encystation and its connection with the expression of the cyst wall protein 1 (CWP1) | Alvarado ME, Chaparro-Gutierrez JJ, ..., Wasserman M | 10.1016/j.actatropica.2021.106183 |
| 35720124 | Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor | Hameed DS, Ovaa H, van der Heden van Noort GJ, Sapmaz A | 10.3389/fmolb.2022.866467 |
| 35477760 | USP14-regulated allostery of the human proteasome by time-resolved cryo-EM | Zhang S, Zou S, ..., Mao Y | 10.1038/s41586-022-04671-8 |
| 35489684 | A ubiquitinome analysis to study the functional roles of the proteasome associated deubiquitinating enzymes USP14 and UCH37 | van der Wal L, Bezstarosti K, Demmers JAA | 10.1016/j.jprot.2022.104592 |
| 33729481 | Proteasome in action: substrate degradation by the 26S proteasome | Sahu I, Glickman MH | 10.1042/BST20200382 |
| 36350544 | High-Throughput Assay for Characterizing Rpn11 Deubiquitinase Activity | Xie G, Dong KC, Worden EJ, Martin A | 10.1007/978-1-0716-2803-4_6 |
| 34399578 | Machine Learning-Enabled Pipeline for Large-Scale Virtual Drug Screening | Gupta A, Zhou HX | 10.1021/acs.jcim.1c00710 |
| 34207520 | Small-Molecule Inhibitors Targeting Proteasome-Associated Deubiquitinases | Moon S, Muniyappan S, Lee SB, Lee BH | 10.3390/ijms22126213 |
| 35155172 | Effect of UV Laser Radiation on "Positive Regulation of Telomere Maintenance" inSaccharomyces cerevisiae | Arjmand B, Safari-Alighiarloo N, ..., Vafaee R | 10.34172/jlms.2021.87 |