PF3D7_1368300

Genome location: Pf3D7_13_v3:2,717,791..2,719,445(+)

Genome classification: Core

Function and Localization

Product Description: non-structural maintenance of chromosomes element 1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030915	Smc5-Smc6 complex
Component	GO:0005634	nucleus
Function	GO:0004842	ubiquitin-protein transferase activity
Process	GO:0000724	double-strand break repair via homologous recombination
Process	GO:0006301	postreplication repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.98
Trophozoite	expressed	0.18	0.88
Schizont	possibly expressed	0.05	0.96
Gametocyte	possibly expressed	0.22	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1368300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -3.08 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1144200)

Relative Growth Rate: 0.03 ± 0.07
Confidence: 6.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5L3

"Best" AlphaFill ligand hit: PH2 (2-amino-6-hydroxymethyl-7,8-dihydro-3h-pteridin-4-one, Local RMSD=5.87) with 6JWR (Global RMSD=17.67)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103559

Most Similar Human Ortholog: Q8WV22

TM-align score: 0.74 | RMSD: 2.86

Seq Identity: 0.21 | Length: 204 / 230

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000169189	NSE1 homolog, SMC5-SMC6 complex component

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	21	6	16
disruptive	4	23	19	36
missense	4	23	17	31

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	33	10	24
disruptive	5	65	31	58
missense	4	54	23	33

PlasmoDB Total SNPs: 36

Non-coding: 30 | Synonymous: 2 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 230 | Molecular Weight (kDa): 26.907

UniProt ID(s): C0H5L3

PDB ID(s): None

Isoelectric Point: 8.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011513	Non-structural maintenance of chromosomes element 1
PFam	N/A	N/A
Superfamily	N/A	N/A