PF3D7_1369000 (GPAA1)

Genome location: Pf3D7_13_v3:2,742,329..2,746,396(+)

Genome classification: Core

Function and Localization

Product Description: glycosylphosphatidylinositol anchor attachment 1 protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 8

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0042765	GPI-anchor transamidase complex
Component	GO:0005783	endoplasmic reticulum
Process	GO:0016255	attachment of GPI anchor to protein

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.24	0.88
Trophozoite	possibly expressed	0.26	0.84
Schizont	expressed	0.14	0.91
Gametocyte	not expressed	0.17	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1369000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.007 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1144900)

Relative Growth Rate: 0.18 ± 0.22
Confidence: 4.39

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5L4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_104164

Most Similar Human Ortholog: O43292

TM-align score: 0.77 | RMSD: 4.05

Seq Identity: 0.15 | Length: 553 / 795

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000197858	glycosylphosphatidylinositol anchor attachment 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	53	27	66
disruptive	24	93	58	137
missense	21	92	57	127

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	95	43	58
disruptive	35	195	86	218
missense	26	182	81	153

PlasmoDB Total SNPs: 220

Non-coding: 158 | Synonymous: 33 | Nonsynonymous: 29 | Stop Codon: 0

Protein Information

Protein Length: 795 | Molecular Weight (kDa): 94.673

UniProt ID(s): C0H5L4

PDB ID(s): None

Isoelectric Point: 9.32

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007246	GPI transamidase component Gaa1
PFam	PF04114	GPI transamidase component Gaa1
Superfamily	N/A	N/A