PF3D7_1372400 (ACS4)

Genome location: Pf3D7_13_v3:2,850,369..2,853,482(+)

Genome classification: Core

Function and Localization

Product Description: acyl-CoA synthetase

SignalP Peptide: MSIILTFLALFVFLICVFPCIT

# Transmembrane Domains: 0

EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005783	endoplasmic reticulum
Component	GO:0016020	membrane
Function	GO:0004467	long-chain fatty acid-CoA ligase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.02	0.99
Trophozoite	no data	0.12	0.91
Schizont	no data	0.07	0.95
Gametocyte	no data	0.01	1.00

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1372400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.731 | #Insertions: 2

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)

Relative Growth Rate: 0.41 ± 0.29
Confidence: 3.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H5N0

"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.14) with 6Q2M (Global RMSD=3.81)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.2.1.3	long-chain-fatty-acid-CoA ligase	ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100152

Most Similar Human Ortholog: B7Z3Z9

TM-align score: 0.91 | RMSD: 2.56

Seq Identity: 0.22 | Length: 517 / 863

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000151726	acyl-CoA synthetase long chain family member 1
ENSG00000164398	acyl-CoA synthetase long chain family member 6
ENSG00000197142	acyl-CoA synthetase long chain family member 5
ENSG00000281938	novel transcript

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 103

Non-coding: 20 | Synonymous: 64 | Nonsynonymous: 14 | Stop Codon: 5

Protein Information

Protein Length: 863 | Molecular Weight (kDa): 99.09

UniProt ID(s): C0H5N0

PDB ID(s): None

Isoelectric Point: 8.06

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000873	AMP-dependent synthetase/ligase
InterPro	IPR020845	AMP-binding, conserved site
PFam	PF00501	AMP-dependent synthetase/ligase
Superfamily	SSF56801	N/A