PF3D7_1401500

Genome location: Pf3D7_14_v3:56,497..59,127(-)

Genome classification: Core

Function and Localization

Product Description: esterase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.-.- (Acting on ester bonds.);3.1.1.5 (Lysophospholipase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016020	membrane
Function	GO:0016787	hydrolase activity
Function	GO:0016788	hydrolase activity, acting on ester bonds
Function	GO:0016298	lipase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.08	0.96
Trophozoite	expressed	0.05	0.97
Schizont	possibly expressed	0.02	0.99
Gametocyte	expressed	0.06	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1401500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.133 | MFS: -2.987 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1220300)

Relative Growth Rate: 1.09 ± 0.18
Confidence: 4.84

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM74

"Best" AlphaFill ligand hit: SRT (s,r meso-tartaric acid, Local RMSD=3.07) with 6T5O (Global RMSD=18.41)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.-.-	Ex: 5-phospho-D-xylono-1,4-lactonase	paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester
3.1.1.5	lysophospholipase	NEMSDSEDTAPCMBEGTAPMSFDMSOCaCl2FeCl3xyleneethanolsulfite...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100231

Most Similar Human Ortholog: A0A3B3ITT3

TM-align score: 0.87 | RMSD: 2.36

Seq Identity: 0.21 | Length: 252 / 373

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000074416	monoglyceride lipase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	6	15	26
disruptive	4	3	3	11
missense	4	3	3	7

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	33	13	23
disruptive	5	18	18	80
missense	4	11	13	46

PlasmoDB Total SNPs: 60

Non-coding: 42 | Synonymous: 15 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 373 | Molecular Weight (kDa): 43.737

UniProt ID(s): Q8IM74

PDB ID(s): None

Isoelectric Point: 8.9

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006494	Plasmodium subtelomeric PST-A
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold