PF3D7_1401800 (CK)

Genome location: Pf3D7_14_v3:69,339..74,061(+)

Genome classification: Core

Function and Localization

Product Description: choline kinase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.1.32 (Choline kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:0005829	cytosol
Function	GO:0033265	choline binding
Function	GO:0004103	choline kinase activity
Process	GO:0006656	phosphatidylcholine biosynthetic process
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.13	0.91
Schizont	expressed	0.37	0.75
Gametocyte	expressed	0.24	0.86

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1401800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -2.947 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1040100)

Relative Growth Rate: 0.16 ± 0.06
Confidence: 7.05

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1040100)

Modification: Disrupted | RMgm-2915

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM71

"Best" AlphaFill ligand hit: OPE (phosphoric acid mono-(2-amino-ethyl) ester, Local RMSD=0.18) with 3FI8 (Global RMSD=1.78)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.32	choline kinase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q8IM71	Choline kinase		HOGENOM, OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST	Bisquinolinium derivative
P35790	Choline kinase alpha	Homo sapiens	OMA, OrthoDB, OrthoMCL BLAST	CHEMBL163319Bis-pyridinium cyclophaneCHEMBL125148CHEMBL332621...
Q9Y259	Choline/ethanolamine kinase	Homo sapiens	OrthoDB, OrthoMCL BLAST	CHEMBL5076272CHEMBL5094399CHEMBL5084844CHEMBL5087533...

Orthology Information

Ortholog Group (OrthoMCL): OG6_120902

No human ortholog(s)

Protein Information

Protein Length: 440 | Molecular Weight (kDa): 52.207

UniProt ID(s): Q8IM71

PDB ID(s): 3FI8, 6YXS, 6YXT

Isoelectric Point: 5.16

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF01633	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 231

Non-coding: 210 | Synonymous: 19 | Nonsynonymous: 2 | Stop Codon: 0

Associated Publications

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16626864	Molecular characterization and localization of Plasmodium falciparum cholinekinase.	Choubey V, Guha M, ..., Bandyopadhyay U	10.1016/j.bbagen.2006.03.003
19845508	Comparison of the cellular and biochemical properties of Plasmodium falciparumcholine and ethanolamine kinases.	Alberge B, Gannoun-Zaki L, ..., Cerdan R	10.1042/BJ20091119
27616047	Plasmodium falciparum Choline Kinase Inhibition Leads to a Major Decrease inPhosphatidylethanolamine Causing Parasite Death.	Serran-Aguilera L, Denton H, ..., Hurtado-Guerrero R	10.1038/srep33189
31631203	A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum.	Das D, Krishnan SR, Roy A, Bulusu G	10.1039/c9mo00124g
38427792	Measuring insecticide resistance in a vacuum: exploring next steps to link resistance data with mosquito control efficacy	Lehane A, Parker-Crockett C, ..., Harrington LC	10.1093/jme/tjae029