PF3D7_1402100

Genome location: Pf3D7_14_v3:80,732..83,840(+)

Genome classification: Core

Function and Localization

Product Description: pseudouridine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 4.2.1.70 (Pseudouridylate synthase);5.4.99.25 (tRNA pseudouridine(55) synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0009982	pseudouridine synthase activity
Process	GO:0031119	tRNA pseudouridine synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.11	0.95
Trophozoite	expressed	0.21	0.87
Schizont	expressed	0.03	0.98
Gametocyte	possibly expressed	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1402100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.967 | MFS: -0.51 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1039800)

Relative Growth Rate: 0.95 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1039800)

Modification: Disrupted | RMgm-2913

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM68

"Best" AlphaFill ligand hit: PHB (p-hydroxybenzoic acid, Local RMSD=1.68) with 6KCL (Global RMSD=15.25)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
4.2.1.70	pseudouridylate synthase	tRNAiodoacetamide5-fluorouracilthiol reagents1,10-phenanthrolinep-chloromercuribenzoate1-(Tetrahydro-2-furanyl)-5-fluorouracil
5.4.99.25	tRNA pseudouridine55 synthase

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101927

Most Similar Human Ortholog: Q3MIT2

TM-align score: 0.57 | RMSD: 5.09

Seq Identity: 0.24 | Length: 372 / 879

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000162927	pseudouridine synthase 10

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	19	54	31	49
disruptive	87	189	88	182
missense	48	147	76	140

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	30	100	29	57
disruptive	128	340	111	265
missense	70	266	93	182

PlasmoDB Total SNPs: 184

Non-coding: 39 | Synonymous: 68 | Nonsynonymous: 76 | Stop Codon: 1

Protein Information

Protein Length: 879 | Molecular Weight (kDa): 105.728

UniProt ID(s): Q8IM68

PDB ID(s): None

Isoelectric Point: 8.22

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
InterPro	IPR039894	tRNA pseudouridine synthase Pus10-like
PFam	N/A	N/A
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
12368868	Sequence of Plasmodium falciparum chromosomes 2, 10, 11 and 14.	Gardner MJ, Shallom SJ, ..., Fraser CM	10.1038/nature01094