Product Description: translocation associated membrane protein, putative
SignalP Peptide: N/A
# Transmembrane Domains: 7
EC Numbers: 2.3.1.24 (Sphingosine N-acyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0005789 | endoplasmic reticulum membrane |
| Function | GO:0016410 | N-acyltransferase activity |
| Function | GO:0050291 | sphingosine N-acyltransferase activity |
| Process | GO:0046513 | ceramide biosynthetic process |
| Process | GO:0065002 | intracellular protein transmembrane transport |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | expressed | 0.05 | 0.97 |
| Schizont | possibly expressed | 0.02 | 0.98 |
| Gametocyte | expressed | 0.33 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1403700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.347 | #Insertions: 2
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1107800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1038300)
Modification: Disrupted | RMgm-2905
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IM57
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.24 | sphingosine N-acyltransferase | FTY720SP600125fumonisinTriton X-100fumonisin B1Fumonisin B24,4'-diisothiocyanostilbene-2,2'-disulfonic acid3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100372
Most Similar Human Ortholog: Q8N5B7
TM-align score: 0.71 | RMSD: 3.24
Seq Identity: 0.23 | Length: 285 / 359
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000090661 | ceramide synthase 4 |
| ENSG00000139624 | ceramide synthase 5 |
| ENSG00000143418 | ceramide synthase 2 |
| ENSG00000172292 | ceramide synthase 6 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 26 | 8 | 20 |
| disruptive | 12 | 25 | 9 | 28 |
| missense | 12 | 25 | 9 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 40 | 14 | 38 |
| disruptive | 21 | 53 | 28 | 70 |
| missense | 18 | 39 | 22 | 55 |
PlasmoDB Total SNPs: 146
Non-coding: 126 | Synonymous: 8 | Nonsynonymous: 12 | Stop Codon: 0
Protein Length: 359 | Molecular Weight (kDa): 43.94
UniProt ID(s): Q8IM57
PDB ID(s): None
Isoelectric Point: 9.72
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006634 | TRAM/LAG1/CLN8 homology domain |
| InterPro | IPR016439 | Sphingosine N-acyltransferase Lag1/Lac1-like |
| PFam | PF03798 | TRAM/LAG1/CLN8 homology domain |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 17919451 | Unraveling the components of protein translocation pathway in human malariaparasite Plasmodium falciparum. | Tuteja R | 10.1016/j.abb.2007.08.031 |