About Gene List

PF3D7_1403800 (MISFIT)

Genome location: Pf3D7_14_v3:136,530..141,420(-)

Genome classification: Core

Function and Localization

Product Description: nuclear formin-like protein MISFIT, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring not expressed 0.06 0.97
Trophozoite not expressed 0.12 0.93
Schizont not expressed 0.24 0.86
Gametocyte possibly expressed 0.98 0.54

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1403800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.549 | #Insertions: 7

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1038200)

Modification: Disrupted | RMgm-2904

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM56

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532440

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 12 80 58 112
disruptive 51 205 120 316
missense 35 189 111 287

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 23 172 63 115
disruptive 88 485 219 435
missense 58 407 179 326

PlasmoDB Total SNPs: 181

Non-coding: 34 | Synonymous: 90 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1542 | Molecular Weight (kDa): 182.259

UniProt ID(s): Q8IM56

PDB ID(s): None

Isoelectric Point: 7.89

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily SSF101447 N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
19662167 Paternal effect of the nuclear formin-like protein MISFIT on Plasmodiumdevelopment in the mosquito vector. Bushell ES, Ecker A, ..., Vlachou D 10.1371/journal.ppat.1000539