PF3D7_1406000 (RBM8A)

Genome location: Pf3D7_14_v3:210,252..211,328(+)

Genome classification: Core

Function and Localization

Product Description: RNA-binding protein 8A, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003723	RNA binding
Function	GO:0003729	mRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	possibly expressed	0.18	0.88
Schizont	possibly expressed	0.02	0.98
Gametocyte	expressed	0.10	0.94

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1406000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.56 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1036200)

Relative Growth Rate: 0.19 ± 0.05
Confidence: 7.30

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1036200)

Modification: Disrupted | RMgm-2896

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM34

"Best" AlphaFill ligand hit: GTA (p1-7-methylguanosine-p3-adenosine-5',5'-triphosphate, Local RMSD=0.27) with 6D0Y (Global RMSD=3.72)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102283

Most Similar Human Ortholog: Q9Y5S9

TM-align score: 0.84 | RMSD: 1.80

Seq Identity: 0.47 | Length: 97 / 107

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000265241	RNA binding motif protein 8A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 66

Non-coding: 60 | Synonymous: 6 | Nonsynonymous: 0 | Stop Codon: 0

Protein Information

Protein Length: 107 | Molecular Weight (kDa): 12.531

UniProt ID(s): Q8IM34

PDB ID(s): None

Isoelectric Point: 4.63

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000504	RNA recognition motif domain
InterPro	IPR008111	RNA-binding motif protein 8
InterPro	IPR033744	RBM8, RNA recognition motif
InterPro	IPR035979	RNA-binding domain superfamily
PFam	PF00076	RNA recognition motif domain
Superfamily	SSF54928	RNA-binding domain superfamily