About Gene List

PF3D7_1406200 (SPT6)

Genome location: Pf3D7_14_v3:217,394..227,116(+)

Genome classification: Core

Function and Localization

Product Description: transcription elongation factor SPT6, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Component GO:0008023 transcription elongation factor complex
Component GO:0035327 transcriptionally active chromatin
Function GO:0042393 histone binding
Function GO:0031491 nucleosome binding
Process GO:0042789 mRNA transcription by RNA polymerase II
Process GO:0034728 nucleosome organization
Process GO:0070827 obsolete chromatin maintenance
Process GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter
Process GO:0050684 regulation of mRNA processing
Process GO:0006368 transcription elongation from RNA polymerase II promoter

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring expressed 2.35 0.21
Trophozoite expressed 2.06 0.16
Schizont expressed 1.04 0.48
Gametocyte possibly expressed 1.57 0.29

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1406200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -2.866 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_129088

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 25 153 80 180
disruptive 114 379 206 506
missense 49 271 159 365

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 52 292 127 190
disruptive 252 784 354 831
missense 110 540 259 499

PlasmoDB Total SNPs: 367

Non-coding: 59 | Synonymous: 178 | Nonsynonymous: 130 | Stop Codon: 0

Protein Information

Protein Length: 2820 | Molecular Weight (kDa): 338.381

UniProt ID(s): Q8IM32

PDB ID(s): None

Isoelectric Point: 4.89

Protein Domain Annotations:

Source Family ID Description
InterPro IPR017072 Transcription elongation factor Spt6
InterPro IPR032706 Transcription elongation factor Spt6, helix-hairpin-helix motif
InterPro IPR035019 Spt6, SH2 domain, N terminus
PFam PF14635 Transcription elongation factor Spt6, helix-hairpin-helix motif
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
38407959 A fluorescent assay for cryptic transcription in Saccharomyces cerevisiae revealsnovel insights into factors that stabilize chromatin structure on newlyreplicated DNA Gao E, Brown JAR, Jung S, Howe LJ 10.1093/genetics/iyae016
36928138 Spt6 directly interacts with Cdc73 and is required for Paf1 complex occupancy atactive genes in Saccharomyces cerevisiae Ellison MA, Namjilsuren S, ..., Arndt KM 10.1093/nar/gkad180
37923091 Genome-wide regulation of Pol II, FACT, and Spt6 occupancies by RSC inSaccharomyces cerevisiae Biernat E, Verma M, Govind CK 10.1016/j.gene.2023.147959