Product Description: glycerophosphodiester phosphodiesterase
SignalP Peptide: MIYFLLFICIVSLLKT
# Transmembrane Domains: 0
EC Numbers: 3.1.4.46 (Glycerophosphodiester phosphodiesterase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0020020 | food vacuole |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0008889 | glycerophosphodiester phosphodiesterase activity |
| Function | GO:0008081 | phosphoric diester hydrolase activity |
| Process | GO:0046475 | glycerophospholipid catabolic process |
| Process | GO:0006644 | phospholipid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.37 | 0.83 |
| Trophozoite | expressed | 1.03 | 0.49 |
| Schizont | expressed | 0.25 | 0.85 |
| Gametocyte | not expressed | 0.17 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1406300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.139 | MFS: -3.15 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IM31
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.4.46 | glycerophosphodiester phosphodiesterase | EDTAphosphatep-nitrophenyl phosphateglycerophosphoethanolamine |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 21 | 16 | 34 |
| disruptive | 11 | 63 | 27 | 78 |
| missense | 11 | 62 | 26 | 73 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 43 | 24 | 33 |
| disruptive | 17 | 112 | 55 | 104 |
| missense | 14 | 105 | 51 | 75 |
PlasmoDB Total SNPs: 135
Non-coding: 92 | Synonymous: 30 | Nonsynonymous: 13 | Stop Codon: 0
Protein Length: 475 | Molecular Weight (kDa): 56.317
UniProt ID(s): Q8IM31
PDB ID(s): None
Isoelectric Point: 5.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily |
| InterPro | IPR030395 | Glycerophosphodiester phosphodiesterase domain |
| PFam | PF03009 | Glycerophosphodiester phosphodiesterase domain |
| Superfamily | SSF51695 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21766240 | Active site similarity between human and Plasmodium falciparumphosphodiesterases: considerations for antimalarial drug design. | Howard BL, Thompson PE, Manallack DT | 10.1007/s10822-011-9458-5 |
| 23000576 | Characterization of a glycerophosphodiesterase with an unusual tripartitedistribution and an important role in the asexual blood stages of Plasmodiumfalciparum. | Denloye T, Dalal S, Klemba M | 10.1016/j.molbiopara.2012.09.004 |
| 38335754 | Biochemical evaluation and ligand binding studies on glycerophosphodiesterphosphodiesterase from Staphylococcus aureus using STD-NMR spectroscopy andmolecular docking analysis | Salar U, Atia-Tul-Wahab, Iqbal Choudhary M | 10.1016/j.bioorg.2024.107153 |
| 36576255 | A choline-releasing glycerophosphodiesterase essential for phosphatidylcholinebiosynthesis and blood stage development in the malaria parasite | Ramaprasad A, Burda PC, ..., Blackman MJ | 10.7554/eLife.82207 |