PF3D7_1406400 (PPR1)

Genome location: Pf3D7_14_v3:230,607..232,875(+)

Genome classification: Core

Function and Localization

Product Description: pentatricopeptide repeat-containing protein 1

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0003723	RNA binding
Function	GO:1990825	sequence-specific mRNA binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	possibly expressed	0.52	0.69
Schizont	not expressed	0.25	0.83
Gametocyte	not expressed	0.27	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1406400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.198 | MFS: -3.327 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1035800)

Relative Growth Rate: 0.16 ± 0.07
Confidence: 6.68

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM30

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_117842

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	26	14	33
disruptive	6	45	28	82
missense	5	41	27	74

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	15	57	20	33
disruptive	17	106	62	112
missense	14	80	53	100

PlasmoDB Total SNPs: 59

Non-coding: 40 | Synonymous: 10 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 608 | Molecular Weight (kDa): 71.529

UniProt ID(s): Q8IM30

PDB ID(s): None

Isoelectric Point: 8.74

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002885	Pentatricopeptide repeat
PFam	PF01535	Pentatricopeptide repeat
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895
31454137	An essential pentatricopeptide repeat protein in the apicomplexan remnantchloroplast.	Hicks JL, Lassadi I, ..., Nisbet RER	10.1111/cmi.13108