PF3D7_1406500 (WDR65)

Genome location: Pf3D7_14_v3:233,001..237,744(-)

Genome classification: Core

Function and Localization

Product Description: WD repeat-containing protein 65, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.03	0.99
Trophozoite	not expressed	0.12	0.93
Schizont	not expressed	0.10	0.94
Gametocyte	not expressed	0.47	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1406500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.636 | #Insertions: 5

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM29

"Best" AlphaFill ligand hit: FAR (farnesyl, Local RMSD=0.28) with 6K1Z (Global RMSD=31.07)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102663

Most Similar Human Ortholog: E9PRV3

TM-align score: 0.88 | RMSD: 1.89

Seq Identity: 0.22 | Length: 164 / 1527

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000243710	cilia and flagella associated protein 57

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	12	81	47	112
disruptive	35	144	97	213
missense	28	130	95	189

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	27	153	60	134
disruptive	78	327	173	353
missense	49	272	142	287

PlasmoDB Total SNPs: 164

Non-coding: 51 | Synonymous: 72 | Nonsynonymous: 41 | Stop Codon: 0

Protein Information

Protein Length: 1527 | Molecular Weight (kDa): 181.534

UniProt ID(s): Q8IM29

PDB ID(s): None

Isoelectric Point: 8.01

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	N/A	N/A
Superfamily	SSF50978	WD40-repeat-containing domain superfamily
Superfamily	SSF69322