Product Description: plasmepsin IV
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.4.23.- (Aspartic endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0004190 | aspartic-type endopeptidase activity |
| Process | GO:0042540 | hemoglobin catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.10 | 0.52 |
| Trophozoite | expressed | 0.70 | 0.61 |
| Schizont | expressed | 0.21 | 0.87 |
| Gametocyte | possibly expressed | 0.63 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1407800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.823 | #Insertions: 4
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1034400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1034400)
Modification: Disrupted | RMgm-2883
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IM16
"Best" AlphaFill ligand hit: CPS (chaps, Local RMSD=0.18) with 7VE0 (Global RMSD=5.92)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.23.- | Ex: renin | I2YYKCNSDSIAANBSDANEDTAPI-3DMSOPCMBTLCK... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8IM16 | Plasmepsin IV [1-448] | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | phenyl-substituted sulfonamides, 6dphenyl-substituted sulfonamides, 6eCHEMBL588662US9353089, 117... | |
| O60989 | Plasmepsin IV, putative | Plasmodium vivax | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL575115CHEMBL584189CHEMBL575150CHEMBL584190... |
| P00797 | Renin | Homo sapiens | OMA, OrthoDB | 2-aminoquinazoline, 3aCGP 38560Renin nonpeptide inhibitor, 2Renin nonpeptide inhibitor, 3... |
| P06281 | Renin-1 | Mus musculus | OMA, OrthoDB | CHEMBL318242CHEMBL321937CHEMBL97865CHEMBL98582... |
| P08424 | Renin | Rattus norvegicus | OMA, OrthoDB | CHEMBL357253CHEMBL414795CHEMBL265833CHEMBL412950... |
| P14091 | Cathepsin E | OrthoDB | US9242943, 36CHEMBL588662US9353089, 117US9353089, 176... | |
| Q6DLW5 | Renin | Macaca mulatta | OrthoDB | CHEMBL1276271Aliskiren HemifumarateIMARIKIREN |
| P07339 | Cathepsin D | Homo sapiens | OrthoDB | Statine-like inhibitor AStatine-like inhibitor 14Statine-like inhibitor 15Statine-like inhibitor 16... |
| P0DJD9 | Pepsin A-5 | Homo sapiens | OrthoDB | CHEMBL326831CHEMBL432424Boc-Phe-His-Leu-CF3-Ile-AmpBoc-Phe-His-Leu-CF3-Ile-Amp... |
| P20142 | Gastricsin | Homo sapiens | OrthoDB | CHEMBL313773CHEMBL418948CHEMBL287423CHEMBL62380... |
| P04073 | Gastricsin | Rattus norvegicus | OrthoDB | CHEMBL28888CHEMBL424222CHEMBL28738 |
| P46925 | Plasmepsin II | Plasmodium falciparum | OrthoMCL BLAST | Statine-like inhibitor AStatine-like inhibitor 14Statine-like inhibitor 15Statine-like inhibitor 16... |
| P39898 | Plasmepsin I | Plasmodium falciparum | OrthoMCL BLAST | Statine-like inhibitor AStatine-like inhibitor 14Statine-like inhibitor 15Statine-like inhibitor 16... |
| Q9Y006 | Plasmepsin III | Plasmodium falciparum | OrthoMCL BLAST | KNI-10006 |
Ortholog Group (OrthoMCL): OG6_100536
Most Similar Human Ortholog: P14091
TM-align score: 0.78 | RMSD: 3.04
Seq Identity: 0.29 | Length: 350 / 449
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000117984 | cathepsin D |
| ENSG00000131400 | napsin A aspartic peptidase |
| ENSG00000196188 | cathepsin E |
| ENSG00000250644 | novel protein |
Protein Length: 449 | Molecular Weight (kDa): 51.046
UniProt ID(s): Q17SB2, Q17SB3, Q17SB5, Q8IM16
PDB ID(s): 1LS5
Isoelectric Point: 5.19
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001461 | Aspartic peptidase A1 family |
| InterPro | IPR001969 | Aspartic peptidase, active site |
| InterPro | IPR021109 | Aspartic peptidase domain superfamily |
| InterPro | IPR033121 | Peptidase family A1 domain |
| InterPro | IPR034164 | Pepsin-like domain |
| PFam | PF00026 | Peptidase family A1 domain |
| Superfamily | SSF50630 | Aspartic peptidase domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 185
Non-coding: 166 | Synonymous: 11 | Nonsynonymous: 7 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11782538 | Four plasmepsins are active in the Plasmodium falciparum food vacuole, includinga protease with an active-site histidine. | Banerjee R, Liu J, ..., Goldberg DE | 10.1073/pnas.022630099 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 27599854 | Crystal structure of Plasmodium falciparum proplasmepsin IV: the plasticity ofproplasmepsins. | Recacha R, Jaudzems K, ..., Tars K | 10.1107/S2053230X16011663 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |