Product Description: FANCJ-like helicase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase);3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0003678 | DNA helicase activity |
| Function | GO:0070182 | DNA polymerase binding |
| Process | GO:0032508 | DNA duplex unwinding |
| Process | GO:0045910 | negative regulation of DNA recombination |
| Process | GO:1904430 | negative regulation of t-circle formation |
| Process | GO:0010569 | regulation of double-strand break repair via homologous recombination |
| Process | GO:0090657 | telomeric loop disassembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.13 | 0.93 |
| Trophozoite | expressed | 0.46 | 0.73 |
| Schizont | possibly expressed | 0.34 | 0.78 |
| Gametocyte | not expressed | 0.77 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1408400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.996 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1034100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1034100)
Modification: Disrupted | RMgm-2881
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IM12
"Best" AlphaFill ligand hit: ACO (acetyl coenzyme *a, Local RMSD=5.37) with 7B3A (Global RMSD=13.32)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9BX63 | Fanconi anemia group J protein | OrthoDB | Telomestatin (TMS)Telomestatin (TMS)Telomestatin (TMS)TMPyP4... |
Ortholog Group (OrthoMCL): OG6_102317
Most Similar Human Ortholog: D6RA96
TM-align score: 0.83 | RMSD: 3.51
Seq Identity: 0.30 | Length: 704 / 1160
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000026036 | RTEL1-TNFRSF6B readthrough (NMD candidate) |
| ENSG00000258366 | regulator of telomere elongation helicase 1 |
Protein Length: 1160 | Molecular Weight (kDa): 135.546
UniProt ID(s): Q8IM12
PDB ID(s): None
Isoelectric Point: 9.15
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006554 | Helicase-like, DEXD box c2 type |
| InterPro | IPR006555 | ATP-dependent helicase, C-terminal |
| InterPro | IPR010614 | DEAD2 |
| InterPro | IPR013020 | ATP-dependent helicase Rad3/Chl1-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF06733 | DEAD2 |
| PFam | PF13307 | ATP-dependent helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 68 | 48 | 90 |
| disruptive | 11 | 61 | 35 | 146 |
| missense | 9 | 60 | 30 | 127 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 33 | 158 | 72 | 80 |
| disruptive | 28 | 207 | 122 | 319 |
| missense | 14 | 164 | 96 | 185 |
PlasmoDB Total SNPs: 197
Non-coding: 116 | Synonymous: 71 | Nonsynonymous: 10 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |