PF3D7_1408900

Genome location: Pf3D7_14_v3:350,700..352,431(-)

Genome classification: Core

Function and Localization

Product Description: tRNA-dihydrouridine synthase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.3.1.- (With NAD(+) or NADP(+) as acceptor.);1.3.1.89 (tRNA-dihydrouridine(47) synthase (NAD(P)(+)))

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0042781	3'-tRNA processing endoribonuclease activity
Function	GO:0017150	tRNA dihydrouridine synthase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.98
Trophozoite	expressed	0.06	0.96
Schizont	possibly expressed	0.01	0.99
Gametocyte	not expressed	0.03	0.98

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1408900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.542 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1033600)

Relative Growth Rate: 0.99 ± 0.11
Confidence: 5.83

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1033600)

Modification: Disrupted | RMgm-2877

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IM07

"Best" AlphaFill ligand hit: FNR (two electron reduced flavin mononucleotide, Local RMSD=0.89) with 4XP7 (Global RMSD=4.54)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.3.1.-	Ex: 2-haloacrylate reductase	EDTANADPHNADP+UracilAFN-1252cisplatintriclosan2-nonanoneHydroxyureaiodoacetatedipyridamoleaquastatin A...
1.3.1.89	tRNA-dihydrouridine47 synthase [NAD(P)+]	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101867

Most Similar Human Ortholog: J3QLE4

TM-align score: 0.94 | RMSD: 1.69

Seq Identity: 0.36 | Length: 304 / 340

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000169718	dihydrouridine synthase 1 like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	25	11	16
disruptive	3	13	11	26
missense	3	13	11	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	45	15	33
disruptive	3	45	31	59
missense	3	33	27	45

PlasmoDB Total SNPs: 102

Non-coding: 88 | Synonymous: 12 | Nonsynonymous: 2 | Stop Codon: 0

Protein Information

Protein Length: 340 | Molecular Weight (kDa): 40.226

UniProt ID(s): Q8IM07

PDB ID(s): None

Isoelectric Point: 8.27

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001269	tRNA-dihydrouridine synthase
InterPro	IPR018517	tRNA-dihydrouridine synthase, conserved site
InterPro	IPR035587	DUS-like, FMN-binding domain
PFam	PF01207	tRNA-dihydrouridine synthase
Superfamily	SSF51395	N/A