PF3D7_1409900 (CDS)

Genome location: Pf3D7_14_v3:387,792..392,617(+)

Genome classification: Core

Function and Localization

Product Description: cytidine diphosphate-diacylglycerol synthase

SignalP Peptide: N/A

# Transmembrane Domains: 7

EC Numbers: 2.7.7.41 (Phosphatidate cytidylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005789	endoplasmic reticulum membrane
Function	GO:0004605	phosphatidate cytidylyltransferase activity
Process	GO:0008654	phospholipid biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.33	0.84
Trophozoite	expressed	1.02	0.47
Schizont	expressed	0.32	0.80
Gametocyte	expressed	1.01	0.50

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1409900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.185 | MFS: -2.912 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1032600)

Relative Growth Rate: 0.09 ± 0.32
Confidence: 3.68

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILZ6

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.41	phosphatidate cytidylyltransferase	ATPCTPNEMCMPSDSCDPEDTAPCMBdCTPFeSO4CHAPSZWT-12...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101561

Most Similar Human Ortholog: O95674

TM-align score: 0.76 | RMSD: 4.06

Seq Identity: 0.33 | Length: 393 / 667

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000101290	CDP-diacylglycerol synthase 2
ENSG00000163624	CDP-diacylglycerol synthase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	45	19	51
disruptive	24	66	46	82
missense	16	47	37	66

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	20	82	34	57
disruptive	43	159	61	174
missense	24	123	49	95

PlasmoDB Total SNPs: 282

Non-coding: 210 | Synonymous: 35 | Nonsynonymous: 35 | Stop Codon: 2

Protein Information

Protein Length: 667 | Molecular Weight (kDa): 77.99

UniProt ID(s): Q8ILZ6, Q9NIH5

PDB ID(s): None

Isoelectric Point: 10.15

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000374	Phosphatidate cytidylyltransferase
InterPro	IPR016720	Phosphatidate cytidylyltransferase, eukaryota
PFam	PF01148	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
10989148	Characterization of Plasmodium falciparum CDP-diacylglycerol synthase, aproteolytically cleaved enzyme.	Martin D, Gannoun-Zaki L, ..., Vial H	10.1016/s0166-6851(00)00260-7
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
20385136	Plasmodium CDP-DAG synthase: an atypical gene with an essential N-terminalextension.	Shastri S, Zeeman AM, ..., Vial HJ	10.1016/j.ijpara.2010.03.006