PF3D7_1410000 (EMC2)

Genome location: Pf3D7_14_v3:392,855..394,689(-)

Genome classification: Core

Function and Localization

Product Description: ER membrane protein complex subunit 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0072546	EMC complex
Process	GO:0034975	protein folding in endoplasmic reticulum

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.55	0.74
Trophozoite	not expressed	0.69	0.61
Schizont	not expressed	0.19	0.87
Gametocyte	not expressed	1.08	0.49

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1410000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.275 | MFS: -3.422 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILZ5

"Best" AlphaFill ligand hit: C2E (c-di-gmp, Local RMSD=2.98) with 4URS (Global RMSD=15.89)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102350

Most Similar Human Ortholog: E5RGJ2

TM-align score: 0.74 | RMSD: 2.05

Seq Identity: 0.20 | Length: 100 / 292

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000104412	ER membrane protein complex subunit 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	13	12	18
disruptive	2	24	16	51
missense	2	24	15	42

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	27	18	23
disruptive	8	64	47	96
missense	5	55	44	61

PlasmoDB Total SNPs: 83

Non-coding: 63 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 292 | Molecular Weight (kDa): 34.953

UniProt ID(s): Q8ILZ5

PDB ID(s): None

Isoelectric Point: 8.79

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR011990	Tetratricopeptide-like helical domain superfamily
InterPro	IPR039856	ER membrane protein complex subunit 2-like
PFam	N/A	N/A
Superfamily	SSF48452	Tetratricopeptide-like helical domain superfamily