PF3D7_1410100

Genome location: Pf3D7_14_v3:395,877..398,406(+)

Genome classification: Core

Function and Localization

Product Description: alpha/beta hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.03	0.99
Trophozoite	expressed	0.03	0.98
Schizont	possibly expressed	0.02	0.99
Gametocyte	expressed	0.65	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1410100

Resistome Missense Mutations: V318L

Resistome Compounds with Missense Mutations: MMV024101

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.168 | MFS: -2.717 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1032400)

Relative Growth Rate: 1.08 ± 0.11
Confidence: 5.77

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1032400)

Modification: Disrupted | RMgm-2869

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILZ4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_112275

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	24	13	29
disruptive	6	28	19	48
missense	4	22	17	40

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	50	15	23
disruptive	12	70	39	89
missense	4	53	32	59

PlasmoDB Total SNPs: 177

Non-coding: 161 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 349 | Molecular Weight (kDa): 41.484

UniProt ID(s): Q8ILZ4

PDB ID(s): None

Isoelectric Point: 6.95

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000073	Alpha/beta hydrolase fold-1
InterPro	IPR022742	Serine aminopeptidase, S33
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF12146	Serine aminopeptidase, S33
Superfamily	SSF53474	Alpha/Beta hydrolase fold

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
28351685	Transcript and protein expression analysis of proteases in the blood stages ofPlasmodium falciparum.	Weissbach T, Golzmann A, ..., Julius Ngwa C	10.1016/j.exppara.2017.03.006