Product Description: rhoptry-associated protein 1
SignalP Peptide: MSFYLGSLVIIFHVLFRNVADG
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Component | GO:0020008 | rhoptry |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.19 | 0.90 |
| Schizont | expressed | 3.35 | 0.12 |
| Gametocyte | possibly expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1410400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.245 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1032100)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1032100)
Modification: Tagged | RMgm-4718
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILZ1
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 14 | 29 |
| disruptive | 31 | 39 | 23 | 81 |
| missense | 30 | 37 | 23 | 69 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 49 | 27 | 43 |
| disruptive | 41 | 112 | 51 | 149 |
| missense | 34 | 94 | 41 | 93 |
PlasmoDB Total SNPs: 154
Non-coding: 107 | Synonymous: 18 | Nonsynonymous: 29 | Stop Codon: 0
Protein Length: 782 | Molecular Weight (kDa): 90.053
UniProt ID(s): D7NSU3, D7NSU5, D7NSU7, D7NSU8, D7NSV5, D7NSV6, D7NSV7, Q25875, Q26007, Q8ILZ1, Q9U431
PDB ID(s): None
Isoelectric Point: 7.08
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR009864 | Rhoptry-associated protein 1, plasmodium |
| PFam | PF07218 | Rhoptry-associated protein 1, plasmodium |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2200961 | Characterisation and sequence of a protective rhoptry antigen from Plasmodiumfalciparum. | Ridley RG, Takacs B, ..., Scaife JG | 10.1016/0166-6851(90)90103-s |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19266084 | Identification of rhoptry trafficking determinants and evidence for a novelsorting mechanism in the malaria parasite Plasmodium falciparum. | Richard D, Kats LM, ..., Coppel RL | 10.1371/journal.ppat.1000328 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 25573429 | A role for adaptor protein complex 1 in protein targeting to rhoptry organellesin Plasmodium falciparum. | Kaderi Kibria KM, Rawat K, ..., Malhotra P | 10.1016/j.bbamcr.2014.12.030 |
| 28205319 | The Plasmodium rhoptry associated protein complex is important forparasitophorous vacuole membrane structure and intraerythrocytic parasite growth | Ghosh S, Kennedy K, ..., de Koning-Ward TF | 10.1111/cmi.12733 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |