Genome location: Pf3D7_14_v3:425,584..429,101(+)
Genome classification: Core
Product Description: eukaryotic translation initiation factor 2 subunit gamma, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.5.3 (Protein-synthesizing GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005850 | eukaryotic translation initiation factor 2 complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0005525 | GTP binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0003743 | translation initiation factor activity |
| Process | GO:0001731 | formation of translation preinitiation complex |
| Process | GO:0045903 | positive regulation of translational fidelity |
| Process | GO:0006413 | translational initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.40 | 0.80 |
| Trophozoite | expressed | 0.57 | 0.67 |
| Schizont | expressed | 0.17 | 0.88 |
| Gametocyte | expressed | 0.20 | 0.88 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1410600
Resistome Missense Mutations: G425D
Resistome Compounds with Missense Mutations: Bortezomib
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.97 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILY9
"Best" AlphaFill ligand hit: PPV (pyrophosphate, Local RMSD=0.04) with 2PMD (Global RMSD=3.34)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.3 | protein-synthesizing GTPase | GDPITPEDTANH4ClppGppneamineGE2270AvanadateviomycinGTPgammaSspermidineneomycin B... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P45975 | Histone-lysine N-methyltransferase Su(var)3-9 | Drosophila melanogaster | OrthoDB | chaetocinchaetocinchaetocin |
Ortholog Group (OrthoMCL): OG6_101121
Most Similar Human Ortholog: Q2VIR3
TM-align score: 0.98 | RMSD: 1.13
Seq Identity: 0.67 | Length: 463 / 465
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130741 | eukaryotic translation initiation factor 2 subunit gamma |
| ENSG00000180574 | eukaryotic translation initiation factor 2 subunit gamma B |
Protein Length: 465 | Molecular Weight (kDa): 51.245
UniProt ID(s): Q8ILY9
PDB ID(s): None
Isoelectric Point: 7.95
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR004161 | Translation elongation factor EFTu-like, domain 2 |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR009001 | Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal |
| InterPro | IPR015256 | Translation initiation factor 2, gamma subunit, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| PFam | PF03144 | Translation elongation factor EFTu-like, domain 2 |
| PFam | PF09173 | Translation initiation factor 2, gamma subunit, C-terminal |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF50465 | Translation elongation factor EF1A/initiation factor IF2gamma, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 28 | 16 | 23 |
| disruptive | 2 | 4 | 1 | 11 |
| missense | 2 | 4 | 1 | 8 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 61 | 19 | 22 |
| disruptive | 2 | 19 | 18 | 49 |
| missense | 2 | 14 | 13 | 30 |
PlasmoDB Total SNPs: 141
Non-coding: 125 | Synonymous: 15 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27303372 | Identification of Plasmodium falciparum Translation Initiation eIF2beta Subunit:Direct Interaction with Protein Phosphatase Type 1. | Tellier G, Lenne A, ..., Khalife J | 10.3389/fmicb.2016.00777 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 37507029 | Binding of human Cdc123 to eIF2gamma | Cardenal Peralta C, Vandroux P, ..., Schmitt E | 10.1016/j.jsb.2023.108006 |
| 37176068 | Human Tissues Exhibit Diverse Composition of Translation Machinery | Anisimova AS, Kolyupanova NM, ..., Dmitriev SE | 10.3390/ijms24098361 |
| 35031321 | Stepwise assembly of the eukaryotic translation initiation factor 2 complex | Vanselow S, Neumann-Arnold L, Wojciech-Moock F, Seufert W | 10.1016/j.jbc.2022.101583 |
| 34796993 | A C-term truncated EIF2Bgamma protein encoded by an intronically polyadenylatedisoform introduces unfavorable EIF2Bgamma-EIF2gamma interactions | Circir A, Koksal Bicakci G, ..., Erson-Bensan AE | 10.1002/prot.26284 |