PF3D7_1411200 (ROM8)

Genome location: Pf3D7_14_v3:451,761..456,500(-)

Genome classification: Core

Function and Localization

Product Description: rhomboid protease ROM8

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.4.21.105 (Rhomboid protease)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	1.36	0.41
Trophozoite	expressed	0.75	0.59
Schizont	expressed	1.16	0.47
Gametocyte	expressed	0.64	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1411200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.131 | MFS: -3.135 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1031300)

Relative Growth Rate: 0.10 ± 0.30
Confidence: 3.79

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILY3

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.21.105	rhomboid protease	dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533440

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	38	19	46
disruptive	5	47	34	70
missense	4	42	32	55

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	77	27	62
disruptive	26	120	73	143
missense	14	89	60	102

PlasmoDB Total SNPs: 276

Non-coding: 240 | Synonymous: 26 | Nonsynonymous: 10 | Stop Codon: 0

Protein Information

Protein Length: 738 | Molecular Weight (kDa): 86.782

UniProt ID(s): Q8ILY3

PDB ID(s): None

Isoelectric Point: 9.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002610	Peptidase S54, rhomboid
InterPro	IPR022764	Peptidase S54, rhomboid domain
PFam	PF01694	Peptidase S54, rhomboid domain
Superfamily	SSF144091	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21