PF3D7_1411900

Genome location: Pf3D7_14_v3:479,713..482,052(+)

Genome classification: Core

Function and Localization

Product Description: p1/s1 nuclease, putative

SignalP Peptide: MSNIFVIFCSLLILIFTKRCSG

# Transmembrane Domains: 0

EC Numbers: 3.1.30.1 (Aspergillus nuclease S(1))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071944	cell periphery
Function	GO:0004519	endonuclease activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.80	0.63
Trophozoite	expressed	0.28	0.83
Schizont	possibly expressed	0.11	0.93
Gametocyte	expressed	0.12	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1411900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.998 | MFS: 0.58 | #Insertions: 3

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1033000)

Modification: Tagged | RMgm-1515

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILX6

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.56) with 6MW7 (Global RMSD=18.99)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.30.1	Aspergillus nuclease S1	KClSDSEDTAEGTAMgCl25'-AMP5'-GMP5'-ADPKH2PO45'-ATPcitrateheparin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101818

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	10	6	23
disruptive	6	22	14	34
missense	6	22	14	30

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	23	14	26
disruptive	10	60	29	76
missense	9	51	22	49

PlasmoDB Total SNPs: 80

Non-coding: 71 | Synonymous: 3 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 327 | Molecular Weight (kDa): 39.05

UniProt ID(s): Q8ILX6

PDB ID(s): None

Isoelectric Point: 8.39

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003154	S1/P1 nuclease
InterPro	IPR008947	Phospholipase C/P1 nuclease domain superfamily
PFam	PF02265	S1/P1 nuclease
Superfamily	SSF48537	Phospholipase C/P1 nuclease domain superfamily

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
27503796	Proteome mapping of Plasmodium: identification of the P. yoelii remodellome.	Siau A, Huang X, ..., Preiser PR	10.1038/srep31055