Product Description: p1/s1 nuclease, putative
SignalP Peptide: MIILFLFCISVFINIIAS
# Transmembrane Domains: 1
EC Numbers: 3.1.30.1 (Aspergillus nuclease S(1))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0045177 | apical part of cell |
| Function | GO:0004519 | endonuclease activity |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.01 | 1.00 |
| Schizont | expressed | 0.51 | 0.70 |
| Gametocyte | not expressed | 0.01 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1412000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.907 | MFS: -2.677 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILX4
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.30.1 | Aspergillus nuclease S1 | KClSDSEDTAEGTAMgCl25'-AMP5'-GMP5'-ADPKH2PO45'-ATPcitrateheparin... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P09598 | Phospholipase C | Bacillus cereus | OrthoMCL BLAST | CHEMBL1288190CHEMBL1288247CHEMBL1288274CHEMBL1288300... |
Protein Length: 320 | Molecular Weight (kDa): 37.803
UniProt ID(s): Q8ILX4
PDB ID(s): None
Isoelectric Point: 9.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003154 | S1/P1 nuclease |
| InterPro | IPR008947 | Phospholipase C/P1 nuclease domain superfamily |
| PFam | PF02265 | S1/P1 nuclease |
| Superfamily | SSF48537 | Phospholipase C/P1 nuclease domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 11 | 6 | 26 |
| disruptive | 4 | 15 | 8 | 37 |
| missense | 4 | 15 | 8 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 26 | 17 | 21 |
| disruptive | 13 | 49 | 22 | 79 |
| missense | 10 | 41 | 17 | 55 |
PlasmoDB Total SNPs: 108
Non-coding: 91 | Synonymous: 8 | Nonsynonymous: 9 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 20037583 | Transcriptional profiling of growth perturbations of the human malaria parasitePlasmodium falciparum. | Hu G, Cabrera A, ..., Bozdech Z | 10.1038/nbt.1597 |