PF3D7_1412400

Genome location: Pf3D7_14_v3:493,932..505,187(-)

Genome classification: Core

Function and Localization

Product Description: conserved Plasmodium protein, unknown function

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: 2.7.1.150 (1-phosphatidylinositol-3-phosphate 5-kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0010008	endosome membrane
Component	GO:0005739	mitochondrion
Function	GO:0000285	1-phosphatidylinositol-3-phosphate 5-kinase activity
Process	GO:0046854	phosphatidylinositol phosphate biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.76	0.66
Trophozoite	not expressed	1.43	0.36
Schizont	not expressed	0.76	0.65
Gametocyte	not expressed	1.63	0.29

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1412400

Resistome Missense Mutations: K720Q

Resistome Compounds with Missense Mutations: BI-2536

Resistome # Samples with Disruptive Mutations: 3 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.917 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.1.150	1-phosphatidylinositol-3-phosphate 5-kinase	YM201636apilimodvacuolin-1

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q9Y2I7	1-phosphatidylinositol 3-phosphate 5-kinase		OrthoDB	CHEMBL4446338CHEMBL2334586APILIMODCHEMBL2178735...
O14986	Phosphatidylinositol 4-phosphate 5-kinase type-1 beta		OrthoDB	CHEMBL203535CHEMBL5171272CHEMBL5206569CHEMBL5191668
Q99755	Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha	Homo sapiens	OrthoDB	CHEMBL203535CHEMBL5171272CHEMBL5206569CHEMBL5191668
O60331	Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma	Homo sapiens	OrthoDB	CHEMBL1645107CHEMBL4640712CHEMBL4303547CHEMBL203535...
P48426	Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha		OrthoDB	CHEMBL4072637CHEMBL4284040CHEMBL4550598CHEMBL4571099...
P78356	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta	Homo sapiens	OrthoDB	ImanixilCHEMBL4533123CHEMBL4439287CHEMBL4446338...
Q8TBX8	Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma	Homo sapiens	OrthoDB	US9623028, Compound 38CHEMBL2205766CHEMBL1529478CHEMBL5083265...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102148

No human ortholog(s)

Protein Information

Protein Length: 3218 | Molecular Weight (kDa): 390.696

UniProt ID(s): Q8ILX0

PDB ID(s): None

Isoelectric Point: 9.56

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002498	Phosphatidylinositol-4-phosphate 5-kinase, core
InterPro	IPR027409	GroEL-like apical domain superfamily
PFam	N/A	N/A
Superfamily	SSF52029	GroEL-like apical domain superfamily
Superfamily	SSF56104

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	31	155	99	193
disruptive	122	449	246	601
missense	69	363	210	513

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	60	319	126	216
disruptive	220	959	427	817
missense	126	751	357	579

PlasmoDB Total SNPs: 706

Non-coding: 161 | Synonymous: 265 | Nonsynonymous: 277 | Stop Codon: 3

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21