Product Description: actin II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005884 | actin filament |
| Component | GO:0005634 | nucleus |
| Function | GO:0000146 | microfilament motor activity |
| Function | GO:0005200 | structural constituent of cytoskeleton |
| Process | GO:0070359 | actin polymerization-dependent cell motility involved in migration of symbiont in host |
| Process | GO:0007010 | cytoskeleton organization |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.20 | 0.90 |
| Trophozoite | expressed | 0.21 | 0.88 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.40 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1412500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.79 | MFS: -2.191 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1459300)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1030100)
Modification: Mutated | RMgm-5278
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILW9
"Best" AlphaFill ligand hit: TAD (beta-methylene-thiazole-4-carboxyamide-adenine dinucleotide, Local RMSD=0.06) with 3BUZ (Global RMSD=1.26)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P60010 | Actin [R335K] | HOGENOM, OrthoDB, OrthoMCL | SCHEMBL1889389Latrunculin ASCHEMBL1889389Latrunculin A... |
Ortholog Group (OrthoMCL): OG6_100127
Most Similar Human Ortholog: P63261
TM-align score: 1.00 | RMSD: 0.45
Seq Identity: 0.76 | Length: 375 / 376
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000075624 | actin beta |
| ENSG00000107796 | actin alpha 2, smooth muscle |
| ENSG00000143632 | actin alpha 1, skeletal muscle |
| ENSG00000159251 | actin alpha cardiac muscle 1 |
| ENSG00000163017 | actin gamma 2, smooth muscle |
| ENSG00000166351 | POTE ankyrin domain family member D |
| ENSG00000169067 | actin beta like 2 |
| ENSG00000183206 | POTE ankyrin domain family member C |
| ENSG00000184009 | actin gamma 1 |
| ENSG00000187537 | POTE ankyrin domain family member G |
| ENSG00000188219 | POTE ankyrin domain family member E |
| ENSG00000196604 | POTE ankyrin domain family member F |
| ENSG00000196834 | POTE ankyrin domain family member I |
| ENSG00000198062 | POTE ankyrin domain family member H |
| ENSG00000222036 | POTE ankyrin domain family member M |
| ENSG00000222038 | POTE ankyrin domain family member J |
| ENSG00000230031 | POTE ankyrin domain family member B2 |
| ENSG00000233917 | POTE ankyrin domain family member B |
| ENSG00000276760 | POTE ankyrin domain family member B-like |
| ENSG00000277630 | POTE ankyrin domain family member D-like |
| ENSG00000278522 | POTE ankyrin domain family member B3 |
| ENSG00000278699 | POTE ankyrin domain family member B3 |
| ENSG00000288113 | POTE ankyrin domain family member B2 |
| ENSG00000288142 | POTE ankyrin domain family member B |
| ENSG00000288347 | POTE ankyrin domain family member B |
Protein Length: 376 | Molecular Weight (kDa): 42.606
UniProt ID(s): A0A144A3N9, Q8ILW9
PDB ID(s): None
Isoelectric Point: 5.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004000 | Actin family |
| InterPro | IPR004001 | Actin, conserved site |
| InterPro | IPR020902 | Actin/actin-like conserved site |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00022 | Actin family |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 29 | 8 | 23 |
| disruptive | 1 | 2 | 2 | 12 |
| missense | 1 | 2 | 2 | 10 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 50 | 10 | 20 |
| disruptive | 1 | 9 | 18 | 81 |
| missense | 1 | 6 | 16 | 56 |
PlasmoDB Total SNPs: 108
Non-coding: 92 | Synonymous: 15 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 2459617 | Extremely diverged actin proteins in Plasmodium falciparum. | Wesseling JG, Smits MA, Schoenmakers JG | 10.1016/0166-6851(88)90107-7 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 24743229 | Structural differences explain diverse functions of Plasmodium actins. | Vahokoski J, Bhargav SP, ..., Kursula I | 10.1371/journal.ppat.1004091 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 34537287 | Gene editing in Plasmodium berghei made easy: Development of a CRISPR/Cas9protocol using linear donor template and ribozymes for sgRNA generation | Deligianni E, Kiamos IS | 10.1016/j.molbiopara.2021.111415 |