Product Description: glycylpeptide N-tetradecanoyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.1.97 (Glycylpeptide N-tetradecanoyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004379 | glycylpeptide N-tetradecanoyltransferase activity |
| Function | GO:0019107 | myristoyltransferase activity |
| Function | GO:0005515 | protein binding |
| Process | GO:0018008 | N-terminal peptidyl-glycine N-myristoylation |
| Process | GO:0006499 | N-terminal protein myristoylation |
| Process | GO:0044409 | entry into host |
| Process | GO:0035891 | exit from host cell |
| Process | GO:0042493 | response to drug |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.21 | 0.86 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.08 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1412800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -3.019 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1029800)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1029800)
Modification: Mutated | RMgm-798
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q9U419
"Best" AlphaFill ligand hit: 6NA (hexanoic acid, Local RMSD=0.37) with 7RK3 (Global RMSD=0.92)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.1.97 | glycylpeptide N-tetradecanoyltransferase | CoAenolaseSC-58272DDD85646histamineCP-014553m-calpainlauric acidL-histidinolCP-030890-27factor NIP71palmitoyl-CoA... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8ILW6 | Glycylpeptide N-tetradecanoyltransferase | Plasmodium falciparum (isolate 3D7) | HOGENOM, OMA, OrthoDB, OrthoMCL BLAST | CHEMBL2171216CHEMBL2171217CHEMBL2171219CHEMBL2171220... |
| P30418 | Glycylpeptide N-tetradecanoyltransferase | Candida albicans | OMA, OrthoDB, OrthoMCL | MLS000774113Non peptidic, 1Non peptidic, 2Non peptidic, 3... |
| Q4Q5S8 | Glycylpeptide N-tetradecanoyltransferase | OMA, OrthoMCL BLAST | US9156811, DDD88557US9156811, DDD99742US9156811, DDD99825US9156811, DDD99830... | |
| Q9UVX3 | Glycylpeptide N-tetradecanoyltransferase | OMA, OrthoDB, OrthoMCL | US9156811, DDD73476US9156811, DDD73490US9156811, DDD85602US9156811, DDD85646... | |
| P14743 | Glycylpeptide N-tetradecanoyltransferase | Saccharomyces cerevisiae | OMA, OrthoDB, OrthoMCL | SC-58272CHEMBL448516 |
| Q388H8 | Glycylpeptide N-tetradecanoyltransferase | OMA, OrthoDB, OrthoMCL, OrthoMCL BLAST | US9156811, DDD16771US9156811, DDD22988US9156811, DDD61495US9156811, DDD64558... | |
| P30419 | Glycylpeptide N-tetradecanoyltransferase 1 | Homo sapiens | OrthoDB, OrthoMCL BLAST | Non peptidic, 4Non peptidic, 5US9156811, DDD73476US9156811, DDD73490... |
| O60551 | Glycylpeptide N-tetradecanoyltransferase 2 | Homo sapiens | OrthoDB, OrthoMCL BLAST | CHEMBL1230468 |
Ortholog Group (OrthoMCL): OG6_101234
Most Similar Human Ortholog: Q5VUC6
TM-align score: 0.93 | RMSD: 1.44
Seq Identity: 0.51 | Length: 387 / 410
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000136448 | N-myristoyltransferase 1 |
| ENSG00000152465 | N-myristoyltransferase 2 |
Protein Length: 410 | Molecular Weight (kDa): 47.97
UniProt ID(s): A0A144A366, Q8ILW6, Q9U419
PDB ID(s): None
Isoelectric Point: 8.24
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000903 | Myristoyl-CoA:protein N-myristoyltransferase |
| InterPro | IPR016181 | Acyl-CoA N-acyltransferase |
| InterPro | IPR022676 | Myristoyl-CoA:protein N-myristoyltransferase, N-terminal |
| InterPro | IPR022677 | Myristoyl-CoA:protein N-myristoyltransferase, C-terminal |
| InterPro | IPR022678 | Myristoyl-CoA:protein N-myristoyltransferase, conserved site |
| PFam | PF01233 | Myristoyl-CoA:protein N-myristoyltransferase, N-terminal |
| PFam | PF02799 | Myristoyl-CoA:protein N-myristoyltransferase, C-terminal |
| Superfamily | SSF55729 | Acyl-CoA N-acyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 16 | 27 |
| disruptive | 1 | 13 | 9 | 28 |
| missense | 1 | 13 | 9 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 44 | 20 | 36 |
| disruptive | 3 | 33 | 20 | 64 |
| missense | 2 | 25 | 19 | 48 |
PlasmoDB Total SNPs: 181
Non-coding: 164 | Synonymous: 12 | Nonsynonymous: 4 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 18973776 | Myristoylated adenylate kinase-2 of Plasmodium falciparum forms a heterodimerwith myristoyltransferase. | Rahlfs S, Koncarevic S, ..., Becker K | https://pubmed.ncbi.nlm.nih.gov/18973776/ |
| 23170970 | Discovery of novel and ligand-efficient inhibitors of Plasmodium falciparum andPlasmodium vivax N-myristoyltransferase. | Rackham MD, Brannigan JA, ..., Leatherbarrow RJ | 10.1021/jm301474t |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24451586 | Validation of N-myristoyltransferase as an antimalarial drug target using anintegrated chemical biology approach. | Wright MH, Clough B, ..., Tate EW | 10.1038/nchem.1830 |
| 25663521 | Homology modeling and molecular dynamics simulation of N-myristoyltransferasefrom Plasmodium falciparum: an insight into novel antimalarial drug design. | Paul P, Chowdhury A, Das Talukdar A, Choudhury MD | 10.1007/s00894-015-2586-4 |
| 34695132 | Inhibition of protein N-myristoylation blocks Plasmodium falciparumintraerythrocytic development, egress and invasion. | Schlott AC, Knuepfer E, ..., Holder AA | 10.1371/journal.pbio.3001408 |
| 38258445 | New thiazolyl-isoxazole derivatives as potential anti-infective agents: design,synthesis, in vitro and in silico antimicrobial efficacy | Bhoye MR, Shinde A, ..., Mhaske PC | 10.1080/07391102.2024.2306497 |