Product Description: ubiquitin-conjugating enzyme E2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme);6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0061631 | ubiquitin conjugating enzyme activity |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0000209 | protein polyubiquitination |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | nd | nd |
| Trophozoite | expressed | nd | nd |
| Schizont | expressed | nd | nd |
| Gametocyte | expressed | nd | nd |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1412900.2
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.964 | MFS: -2.185 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1029700)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1029700)
Modification: Disrupted | RMgm-2851
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILW5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
| 2.3.2.23 | E2 ubiquitin-conjugating enzyme | CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione... |
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102995
Most Similar Human Ortholog: Q96B02
TM-align score: 0.90 | RMSD: 1.52
Seq Identity: 0.51 | Length: 145 / 299
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000104343 | ubiquitin conjugating enzyme E2 W |
| ENSG00000258677 | novel protein, UBE2W-STAU2 readthrough |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 5 | 2 | 5 |
| disruptive | 1 | 8 | 4 | 15 |
| missense | 1 | 8 | 4 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 8 | 4 | 7 |
| disruptive | 3 | 14 | 15 | 26 |
| missense | 3 | 13 | 14 | 14 |
PlasmoDB Total SNPs: 163
Non-coding: 153 | Synonymous: 6 | Nonsynonymous: 4 | Stop Codon: 0
Protein Length: 299 | Molecular Weight (kDa): 34.417
UniProt ID(s): Q8ILW5
PDB ID(s): None
Isoelectric Point: 7.48
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000608 | Ubiquitin-conjugating enzyme E2 |
| InterPro | IPR016135 | Ubiquitin-conjugating enzyme/RWD-like |
| InterPro | IPR018997 | PUB domain |
| InterPro | IPR036339 | PUB-like domain superfamily |
| PFam | PF00179 | Ubiquitin-conjugating enzyme E2 |
| PFam | PF09409 | PUB domain |
| Superfamily | SSF143503 | PUB-like domain superfamily |
| Superfamily | SSF54495 | Ubiquitin-conjugating enzyme/RWD-like |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |