PF3D7_1414000 (RPN13)

Genome location: Pf3D7_14_v3:552,757..555,969(-)

Genome classification: Core

Function and Localization

Product Description: 26S proteasome regulatory subunit RPN13, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0008541	proteasome regulatory particle, lid subcomplex
Function	GO:0061133	endopeptidase activator activity
Function	GO:0070628	proteasome binding
Function	GO:0043130	ubiquitin binding
Process	GO:0006511	ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.07	0.97
Trophozoite	expressed	0.58	0.66
Schizont	expressed	0.45	0.72
Gametocyte	expressed	0.82	0.56

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1414000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.204 | MFS: -3.472 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILV5

"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=3.05) with 7X2Q (Global RMSD=17.51)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102724

Most Similar Human Ortholog: Q16186

TM-align score: 0.49 | RMSD: 4.09

Seq Identity: 0.26 | Length: 156 / 253

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000130706	adhesion regulating molecule 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	13	8	17
disruptive	2	11	15	23
missense	2	11	15	23

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	27	11	18
disruptive	4	38	28	58
missense	3	33	27	48

PlasmoDB Total SNPs: 258

Non-coding: 239 | Synonymous: 15 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 253 | Molecular Weight (kDa): 29.488

UniProt ID(s): Q8ILV5

PDB ID(s): None

Isoelectric Point: 5.26

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006773	Proteasomal ubiquitin receptor Rpn13/ADRM1
PFam	PF04683	Proteasomal ubiquitin receptor Rpn13/ADRM1
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104