Product Description: serine/threonine protein phosphatase PP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.3.16 (Protein-serine/threonine phosphatase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0005515 | protein binding |
| Function | GO:0004722 | protein serine/threonine phosphatase activity |
| Process | GO:0044409 | entry into host |
| Process | GO:0035891 | exit from host cell |
| Process | GO:0006470 | protein dephosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.05 | 0.98 |
| Trophozoite | expressed | 0.28 | 0.82 |
| Schizont | expressed | 0.71 | 0.58 |
| Gametocyte | expressed | 0.23 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1414400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.145 | MFS: -2.53 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1028300)
Modification: Tagged | RMgm-5004
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILV1
"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.11) with 6GHM (Global RMSD=0.33)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.3.16 | protein-serine/threonine phosphatase | ATPADPUDPAMPGTPUTPUMPNaFEDTAEGTAcAMPNADPH... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P36873 | Serine/threonine-protein phosphatase PP1-gamma catalytic subunit | Homo sapiens | HOGENOM, OMA, OrthoDB | CHEMBL200117CHEMBL202197okadaic acidokadaic acid... |
| P67774 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Bos taurus | OrthoDB | CHEMBL318253CHEMBL421377CHEMBL421377CHEMBL318253... |
| P67775 | Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform | Homo sapiens | OrthoDB | okadaic acid analogueokadaic acid |
| P62136 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | Homo sapiens | OrthoDB | MLS001241052 |
Ortholog Group (OrthoMCL): OG6_100222
Most Similar Human Ortholog: P36873
TM-align score: 0.99 | RMSD: 0.53
Seq Identity: 0.84 | Length: 304 / 304
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172531 | protein phosphatase 1 catalytic subunit alpha |
| ENSG00000186298 | protein phosphatase 1 catalytic subunit gamma |
| ENSG00000213639 | protein phosphatase 1 catalytic subunit beta |
Protein Length: 304 | Molecular Weight (kDa): 34.904
PDB ID(s): None
Isoelectric Point: 5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004843 | Calcineurin-like phosphoesterase domain, ApaH type |
| InterPro | IPR006186 | Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase |
| InterPro | IPR031675 | Serine-threonine protein phosphatase, N-terminal |
| InterPro | IPR037979 | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit |
| PFam | PF00149 | Calcineurin-like phosphoesterase domain, ApaH type |
| PFam | PF16891 | Serine-threonine protein phosphatase, N-terminal |
| Superfamily | SSF56300 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 25 | 7 | 26 |
| disruptive | 0 | 1 | 6 | 33 |
| missense | 0 | 1 | 5 | 28 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 39 | 16 | 14 |
| disruptive | 1 | 22 | 28 | 85 |
| missense | 0 | 15 | 20 | 62 |
PlasmoDB Total SNPs: 182
Non-coding: 175 | Synonymous: 7 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16629662 | Regulation of protein phosphatase type 1 and cell cycle progression by PfLRR1, anovel leucine-rich repeat protein of the human malaria parasite Plasmodiumfalciparum. | Daher W, Browaeys E, ..., Khalife J | 10.1111/j.1365-2958.2006.05119.x |
| 22128182 | Plasmodium falciparum inhibitor-3 homolog increases protein phosphatase type 1activity and is essential for parasitic survival. | Freville A, Landrieu I, ..., Khalife J | 10.1074/jbc.M111.276865 |
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 25132288 | Identification of a Plasmodium falciparum inhibitor-2 motif involved in thebinding and regulation activity of protein phosphatase type 1. | Freville A, Tellier G, ..., Landrieu I | 10.1111/febs.12960 |
| 26988354 | Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 inPlasmodium falciparum. | Hollin T, De Witte C, ..., Khalife J | 10.1186/s12864-016-2571-z |
| 27303372 | Identification of Plasmodium falciparum Translation Initiation eIF2beta Subunit:Direct Interaction with Protein Phosphatase Type 1. | Tellier G, Lenne A, ..., Khalife J | 10.3389/fmicb.2016.00777 |
| 30429842 | Characterization of a Protein Phosphatase Type-1 and a Kinase Anchoring Proteinin Plasmodium falciparum. | Lenne A, De Witte C, ..., Pierrot C | 10.3389/fmicb.2018.02617 |
| 31148576 | Plasmodium pseudo-Tyrosine Kinase-like binds PP1 and SERA5 and is exported tohost erythrocytes. | Gnangnon B, Freville A, ..., Pierrot C | 10.1038/s41598-019-44542-3 |
| 32669539 | Co-option of Plasmodium falciparum PP1 for egress from host erythrocytes. | Paul AS, Miliu A, ..., Duraisingh MT | 10.1038/s41467-020-17306-1 |
| 34956931 | Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening ofPlasmodium falciparum. | Hou N, Li S, ..., Chen Q | https://pubmed.ncbi.nlm.nih.gov/34956931/ |
| 38303113 | DNA damage-induced allosteric activation of protein phosphatase PP1:NIPP1 throughSrc kinase-induced circularization of NIPP1 | Wu D, Van der Hoeven G, ..., Bollen M | 10.1111/febs.17064 |
| 38227612 | Phosphoregulation of the yeast Pma1 H+-ATPase autoinhibitory domain involves thePtk1/2 kinases and the Glc7 PP1 phosphatase and is under TORC1 control | Guarini N, Saliba E, Andre B | 10.1371/journal.pgen.1011121 |
| 38199846 | E. hellem Ser/Thr protein phosphatase PP1 targets the DC MAPK pathway and impairsimmune functions | Bao J, Tang Y, ..., Zhou Z | 10.26508/lsa.202302375 |
| 38267954 | Emerging roles of the Protein Phosphatase 1 (PP1) in the context of viralinfections | Corda PO, Bollen M, Ribeiro D, Fardilha M | 10.1186/s12964-023-01468-8 |