Product Description: mRNA-capping enzyme subunit alpha
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.7.50 (mRNA guanylyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0004484 | mRNA guanylyltransferase activity |
| Process | GO:0006370 | 7-methylguanosine mRNA capping |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.67 | 0.69 |
| Trophozoite | expressed | 0.45 | 0.74 |
| Schizont | expressed | 0.54 | 0.67 |
| Gametocyte | expressed | 1.09 | 0.45 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1414600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.211 | MFS: -2.744 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I720
"Best" AlphaFill ligand hit: GMP (guanosine, Local RMSD=0.03) with 4PZ6 (Global RMSD=5.63)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.7.50 | mRNA guanylyltransferase | ATPGTPITPXTPEDTAddGTPGpppAphosphate8-iodoGTP6-thio-GTPdiphosphate8-bromo-GTP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101990
Most Similar Human Ortholog: O60942
TM-align score: 0.58 | RMSD: 3.04
Seq Identity: 0.28 | Length: 330 / 520
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000111880 | RNA guanylyltransferase and 5-phosphatase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 39 | 21 | 32 |
| disruptive | 9 | 38 | 26 | 60 |
| missense | 8 | 35 | 24 | 47 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 60 | 31 | 46 |
| disruptive | 21 | 106 | 58 | 158 |
| missense | 16 | 82 | 41 | 103 |
PlasmoDB Total SNPs: 98
Non-coding: 65 | Synonymous: 21 | Nonsynonymous: 11 | Stop Codon: 1
Protein Length: 520 | Molecular Weight (kDa): 61.154
UniProt ID(s): Q8I720
PDB ID(s): None
Isoelectric Point: 6.33
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001339 | mRNA capping enzyme, catalytic domain |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR013846 | mRNA capping enzyme, C-terminal |
| PFam | PF01331 | mRNA capping enzyme, catalytic domain |
| PFam | PF03919 | mRNA capping enzyme, C-terminal |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF56091 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 11248030 | A yeast-like mRNA capping apparatus in Plasmodium falciparum. | Ho CK, Shuman S | 10.1073/pnas.061636198 |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23280723 | Mutations in the N-terminal region of the Schizosaccharomyces pombe glutathionetransporter pgt1(+) allows functional expression in Saccharomyces cerevisiae | Thakur A, Bachhawat AK | 10.1002/yea.2939 |
| 19589778 | Gln-222 in transmembrane domain 4 and Gln-526 in transmembrane domain 9 arecritical for substrate recognition in the yeast high affinity glutathionetransporter, Hgt1p | Kaur J, Bachhawat AK | 10.1074/jbc.M109.029728 |
| 18662319 | Pgt1, a glutathione transporter from the fission yeast Schizosaccharomyces pombe | Thakur A, Kaur J, Bachhawat AK | 10.1111/j.1567-1364.2008.00423.x |
| 22226946 | The Ubiquitin ligase Ubr11 is essential for oligopeptide utilization in thefission yeast Schizosaccharomyces pombe | Kitamura K, Nakase M, Tohda H, Takegawa K | 10.1128/EC.05253-11 |