PF3D7_1414700

Genome location: Pf3D7_14_v3:592,791..598,162(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin carboxyl-terminal hydrolase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.4.19.12 (Ubiquitinyl hydrolase 1)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.36	0.44
Trophozoite	expressed	0.73	0.61
Schizont	possibly expressed	0.22	0.87
Gametocyte	expressed	1.21	0.42

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1414700

Resistome Missense Mutations: A684T

Resistome Compounds with Missense Mutations: MMV028038

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.594 | #Insertions: 7

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILU9

"Best" AlphaFill ligand hit: AYE (allylamine, Local RMSD=1.07) with 8BS3 (Global RMSD=2.62)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.19.12	ubiquitinyl hydrolase 1	KClNEMDTTEDTAEGTARNAisiRNApeptidesAntibodyubiquitinLDN-574442-propenal...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101457

Most Similar Human Ortholog: K7EMM6

TM-align score: 0.74 | RMSD: 2.93

Seq Identity: 0.29 | Length: 213 / 1384

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000055483	ubiquitin specific peptidase 36
ENSG00000106346	ubiquitin specific peptidase 42

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	77	55	96
disruptive	53	187	115	277
missense	44	166	101	230

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	155	59	100
disruptive	118	420	183	420
missense	80	342	151	297

PlasmoDB Total SNPs: 245

Non-coding: 128 | Synonymous: 60 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1384 | Molecular Weight (kDa): 164.385

UniProt ID(s): Q8ILU9

PDB ID(s): None

Isoelectric Point: 10.04

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001394	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
InterPro	IPR018200	Ubiquitin specific protease, conserved site
InterPro	IPR037798	Ubiquitin carboxyl-terminal hydrolase 8
InterPro	IPR038765	Papain-like cysteine peptidase superfamily
PFam	PF00443	Peptidase C19, ubiquitin carboxyl-terminal hydrolase
Superfamily	SSF54001	Papain-like cysteine peptidase superfamily