Product Description: ATP-dependent protease, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.21.53 (Endopeptidase La)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005782 | peroxisomal matrix |
| Function | GO:0004176 | ATP-dependent peptidase activity |
| Function | GO:0004252 | serine-type endopeptidase activity |
| Process | GO:0016485 | protein processing |
| Process | GO:0006625 | protein targeting to peroxisome |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | expressed | 0.18 | 0.90 |
| Schizont | expressed | 0.05 | 0.97 |
| Gametocyte | possibly expressed | 0.25 | 0.85 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1414900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.129 | MFS: -2.93 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1027800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILU7
"Best" AlphaFill ligand hit: BO2 (bortezomib, Local RMSD=0.35) with 6X27 (Global RMSD=2.44)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.53 | Endopeptidase La | BRENDA SOAP query unsuccessful |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P36776 | Lon protease homolog, mitochondrial | HOGENOM, OMA, OrthoDB | CHEMBL4870013CHEMBL4877126CHEMBL4850826CHEMBL4876920... |
Ortholog Group (OrthoMCL): OG6_100411
Most Similar Human Ortholog: H3BQ67
TM-align score: 0.89 | RMSD: 1.87
Seq Identity: 0.34 | Length: 168 / 1192
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000102910 | lon peptidase 2, peroxisomal |
| ENSG00000196365 | lon peptidase 1, mitochondrial |
Protein Length: 1192 | Molecular Weight (kDa): 141.702
UniProt ID(s): Q8ILU7
PDB ID(s): None
Isoelectric Point: 8.74
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003111 | Lon, substrate-binding domain |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR008269 | Peptidase S16, Lon proteolytic domain |
| InterPro | IPR020568 | Ribosomal protein S5 domain 2-type fold |
| InterPro | IPR027065 | Lon protease |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF02190 | Lon, substrate-binding domain |
| PFam | PF05362 | Peptidase S16, Lon proteolytic domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF54211 | Ribosomal protein S5 domain 2-type fold |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 49 | 48 | 85 |
| disruptive | 39 | 196 | 103 | 258 |
| missense | 28 | 171 | 96 | 221 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 27 | 117 | 59 | 95 |
| disruptive | 78 | 433 | 224 | 435 |
| missense | 50 | 353 | 176 | 284 |
PlasmoDB Total SNPs: 116
Non-coding: 0 | Synonymous: 57 | Nonsynonymous: 57 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |