Product Description: uracil-DNA glycosylase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.2.2.27 (Uracil-DNA glycosylase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004844 | uracil DNA N-glycosylase activity |
| Process | GO:0006284 | base-excision repair |
| Process | GO:0097510 | base-excision repair, AP site formation via deaminated base removal |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.29 | 0.82 |
| Schizont | possibly expressed | 0.13 | 0.91 |
| Gametocyte | expressed | 0.37 | 0.78 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1415000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.13 | MFS: -3.285 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILU6
"Best" AlphaFill ligand hit: URA (uracil, Local RMSD=0.32) with 2EUG (Global RMSD=1.13)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.2.2.27 | uracil-DNA glycosylase | UgiMgCl2UracilG-U dsDNAgentamicin5-AzauracilUgi peptideUgi proteinAlba proteinSso7d proteinUGI inhibitor4-methylindole... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P13051 | Uracil-DNA glycosylase | Homo sapiens | OMA, OrthoDB | CID25210526CHEMBL74742 |
Ortholog Group (OrthoMCL): OG6_101690
Most Similar Human Ortholog: P13051
TM-align score: 0.71 | RMSD: 2.30
Seq Identity: 0.39 | Length: 237 / 322
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000076248 | uracil DNA glycosylase |
Protein Length: 322 | Molecular Weight (kDa): 37.461
UniProt ID(s): Q8ILU6
PDB ID(s): None
Isoelectric Point: 9.78
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002043 | Uracil-DNA glycosylase family 1 |
| InterPro | IPR005122 | Uracil-DNA glycosylase-like |
| InterPro | IPR018085 | Uracil-DNA glycosylase, active site |
| InterPro | IPR036895 | Uracil-DNA glycosylase-like domain superfamily |
| PFam | PF03167 | Uracil-DNA glycosylase-like |
| Superfamily | SSF52141 | Uracil-DNA glycosylase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 20 | 13 | 17 |
| disruptive | 3 | 18 | 14 | 26 |
| missense | 3 | 17 | 14 | 22 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 28 | 19 | 21 |
| disruptive | 8 | 46 | 15 | 58 |
| missense | 4 | 38 | 13 | 39 |
PlasmoDB Total SNPs: 51
Non-coding: 28 | Synonymous: 17 | Nonsynonymous: 5 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24742318 | Molecular characterization of Plasmodium falciparum uracil-DNA glycosylase andits potential as a new anti-malarial drug target. | Suksangpleng T, Leartsakulpanich U, ..., Chavalitshewinkoon-Petmitr P | 10.1186/1475-2875-13-149 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |