PF3D7_1415200

Genome location: Pf3D7_14_v3:611,486..613,217(+)

Genome classification: Core

Function and Localization

Product Description: DNA-directed RNA polymerases I and III subunit RPAC2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.6 (DNA-directed RNA polymerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005736	RNA polymerase I complex
Component	GO:0005666	RNA polymerase III complex
Function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
Process	GO:0006351	transcription, DNA-templated

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	possibly expressed	0.02	0.99
Schizont	expressed	0.01	1.00
Gametocyte	possibly expressed	0.01	0.99

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1415200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.132 | MFS: 0 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILU4

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.6	DNA-directed RNA polymerase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102491

Most Similar Human Ortholog: P0DPB6

TM-align score: 0.86 | RMSD: 1.61

Seq Identity: 0.41 | Length: 87 / 96

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000186184	RNA polymerase I and III subunit D

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	0	0	1
disruptive	0	0	0	0
missense	0	0	0	0

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	0	1	0	0
disruptive	0	0	0	0
missense	0	0	0	0

PlasmoDB Total SNPs: 169

Non-coding: 159 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 96 | Molecular Weight (kDa): 11.005

UniProt ID(s): Q8ILU4

PDB ID(s): None

Isoelectric Point: 4.85

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008193	DNA-directed RNA polymerase Rpb11, 13-16kDa subunit, conserved site
InterPro	IPR009025	DNA-directed RNA polymerase, RBP11-like dimerisation domain
InterPro	IPR033898	DNA-directed RNA polymerases I and III subunit AC19
InterPro	IPR036603	RNA polymerase, RBP11-like subunit
PFam	PF13656	DNA-directed RNA polymerase, RBP11-like dimerisation domain
Superfamily	SSF55257	RNA polymerase, RBP11-like subunit