Product Description: ribosomal RNA small subunit methyltransferase A1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.- (Methyltransferases.);2.1.1.183 (18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0003677 | DNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0000179 | rRNA (adenine-N6,N6-)-dimethyltransferase activity |
| Function | GO:0008649 | rRNA methyltransferase activity |
| Process | GO:0006352 | DNA-templated transcription, initiation |
| Process | GO:0031167 | rRNA methylation |
| Process | GO:0000154 | rRNA modification |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.31 | 0.84 |
| Trophozoite | expressed | 0.21 | 0.87 |
| Schizont | possibly expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1415800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.156 | MFS: -3.214 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1462800)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILT8
"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.61) with 3GRV (Global RMSD=6.11)
BRENDA EC Inhibitors:
Orthology to BindingDB Entries:
Ortholog Group (OrthoMCL): OG6_100569
Most Similar Human Ortholog: A0A7P0T9Y7
TM-align score: 0.95 | RMSD: 1.41
Seq Identity: 0.46 | Length: 283 / 381
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000086189 | DIMT1 rRNA methyltransferase and ribosome maturation factor |
Protein Length: 381 | Molecular Weight (kDa): 43.922
UniProt ID(s): Q8ILT8
PDB ID(s): 2H1R
Isoelectric Point: 9.99
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001737 | Ribosomal RNA adenine methyltransferase KsgA/Erm |
| InterPro | IPR011530 | Ribosomal RNA adenine dimethylase |
| InterPro | IPR020598 | Ribosomal RNA adenine methylase transferase, N-terminal |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF00398 | Ribosomal RNA adenine methyltransferase KsgA/Erm |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 18 | 16 | 24 |
| disruptive | 2 | 18 | 16 | 40 |
| missense | 2 | 17 | 15 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 46 | 16 | 18 |
| disruptive | 4 | 66 | 37 | 75 |
| missense | 3 | 50 | 29 | 40 |
PlasmoDB Total SNPs: 67
Non-coding: 48 | Synonymous: 15 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 29909066 | Characterization of a Plasmodium falciparum rRNA methyltransferase. | Gupta K, Gupta A, Habib S | 10.1016/j.molbiopara.2018.06.001 |
| 32896572 | PfKsgA1 functions as a transcription initiation factor and interacts with theN-terminal region of the mitochondrial RNA polymerase of Plasmodium falciparum. | Gupta A, Shrivastava D, ..., Habib S | 10.1016/j.ijpara.2020.07.010 |