Product Description: metacaspase-3
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.22.- (Cysteine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0008236 | serine-type peptidase activity |
| Process | GO:0006508 | proteolysis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.45 | 0.74 |
| Schizont | expressed | 1.20 | 0.42 |
| Gametocyte | expressed | 0.68 | 0.64 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1416200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.135 | MFS: -2.929 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1026500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1026500)
Modification: Disrupted | RMgm-2843
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILT4
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.22.- | Ex: staphylococcal cysteine proteinase | NEME64SpiSDSCysEDTAPCMBEGTAPMSFTLCKTPCKMENT... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 17 | 130 | 70 | 136 |
| disruptive | 85 | 358 | 204 | 424 |
| missense | 61 | 320 | 188 | 374 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 40 | 230 | 106 | 151 |
| disruptive | 163 | 756 | 328 | 564 |
| missense | 116 | 646 | 283 | 430 |
PlasmoDB Total SNPs: 401
Non-coding: 152 | Synonymous: 132 | Nonsynonymous: 117 | Stop Codon: 0
Protein Length: 2280 | Molecular Weight (kDa): 264.925
UniProt ID(s): Q8ILT4
PDB ID(s): None
Isoelectric Point: 9.73
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | PF00656 | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 31301397 | Metacaspase-3 of Plasmodium falciparum: An atypical trypsin-like serine protease. | Kumar B, Verma S, ..., Pandey KC | 10.1016/j.ijbiomac.2019.07.067 |
| 17375468 | Cysteine proteinases of Trypanosoma cruzi: from digestive enzymes to programmedcell death mediators | Kosec G, Alvarez V, Cazzulo JJ | https://pubmed.ncbi.nlm.nih.gov/17375468/ |
| 16507595 | Bloodstream form Trypanosoma brucei depend upon multiple metacaspases associatedwith RAB11-positive endosomes | Helms MJ, Ambit A, ..., Mottram JC | 10.1242/jcs.02809 |
| 34384491 | Requirement of Toxoplasma gondii metacaspases for IMC1 maturation, endodyogenyand virulence in mice | Li M, Liu J, ..., Liu Q | 10.1186/s13071-021-04878-0 |
| 21949125 | Trypanosoma brucei metacaspase 4 is a pseudopeptidase and a virulence factor | Proto WR, Castanys-Munoz E, ..., Mottram JC | 10.1074/jbc.M111.292334 |