Product Description: N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.4.54 (N-acetylphosphatidylethanolamine-hydrolyzing phospholipase D)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.06 | 0.97 |
| Trophozoite | possibly expressed | 0.41 | 0.75 |
| Schizont | possibly expressed | 0.19 | 0.87 |
| Gametocyte | not expressed | 0.25 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1416300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.205 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0905200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILT3
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.4.54 | N-acetylphosphatidylethanolamine-hydrolysing phospholipase D | DTTEDTAoleatecapsaicinnitrocefinTriton X-100noladin etherphosphatidylhexanolN-oleoylethanolaminereceptor agonist ACEA2-arachidonoylglycerolN-stearoylethanolamine... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q8BH82 | N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D | OMA, OrthoDB | CHEMBL4790197CHEMBL4782800CHEMBL4754918CHEMBL4780517... | |
| Q6IQ20 | N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D | OMA, OrthoDB | CHEMBL4790197CHEMBL4762175CHEMBL4791170CHEMBL4778789... |
Ortholog Group (OrthoMCL): OG6_102519
Most Similar Human Ortholog: Q6IQ20
TM-align score: 0.73 | RMSD: 2.29
Seq Identity: 0.24 | Length: 309 / 417
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000161048 | N-acyl phosphatidylethanolamine phospholipase D |
| ENSG00000275723 | N-acyl phosphatidylethanolamine phospholipase D |
Protein Length: 417 | Molecular Weight (kDa): 49.137
UniProt ID(s): Q8ILT3
PDB ID(s): None
Isoelectric Point: 10.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001279 | Metallo-beta-lactamase |
| InterPro | IPR024884 | N-acyl-phosphatidylethanolamine-hydrolysing phospholipase D |
| InterPro | IPR036866 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| PFam | PF12706 | Metallo-beta-lactamase |
| Superfamily | SSF56281 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 20 | 18 | 35 |
| disruptive | 7 | 51 | 26 | 68 |
| missense | 7 | 51 | 26 | 61 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 50 | 31 | 31 |
| disruptive | 12 | 115 | 49 | 143 |
| missense | 11 | 102 | 42 | 90 |
PlasmoDB Total SNPs: 72
Non-coding: 54 | Synonymous: 11 | Nonsynonymous: 6 | Stop Codon: 1
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |