Product Description: NADP-specific glutamate dehydrogenase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 1.4.1.4 (Glutamate dehydrogenase (NADP(+)))
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0004354 | glutamate dehydrogenase (NADP+) activity |
| Process | GO:0006537 | glutamate biosynthetic process |
| Process | GO:0006536 | glutamate metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.66 | 0.71 |
| Trophozoite | expressed | 0.13 | 0.92 |
| Schizont | expressed | 0.13 | 0.91 |
| Gametocyte | possibly expressed | 0.14 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1416500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.704 | #Insertions: 3
PlasmoGEM Phenotype: Insufficient data (Pb ortholog: PBANKA_1026200)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1026200)
Modification: Disrupted | RMgm-4077
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILT0
"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=0.37) with 3ETD (Global RMSD=4.62)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.4.1.4 | glutamate dehydrogenase (NADP+) | O2ATPADPAMPKClNAD+NADHEDTANH4+NADPHNADP+HgCl2... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P49448 | Glutamate dehydrogenase 2, mitochondrial | OrthoDB | US8604016, 1US8604016, 1US8604016, 295US8604016, 295... | |
| P00366 | Glutamate dehydrogenase 1, mitochondrial | Bos taurus | OrthoDB | [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl ({[(3R,4S)-5-(3-carbamoyl-1,4-dihydropyridin-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphonato}oxy)phosphonateCHEMBL2364562 |
Ortholog Group (OrthoMCL): OG6_100455
Most Similar Human Ortholog: P49448
TM-align score: 0.83 | RMSD: 2.08
Seq Identity: 0.27 | Length: 415 / 470
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000148672 | glutamate dehydrogenase 1 |
| ENSG00000182890 | glutamate dehydrogenase 2 |
| ENSG00000288118 | glutamate dehydrogenase 2 |
Protein Length: 470 | Molecular Weight (kDa): 52.546
UniProt ID(s): O96940, Q8ILT0, Q9GTK5, Q9TXS8
PDB ID(s): None
Isoelectric Point: 7.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006095 | Glutamate/phenylalanine/leucine/valine dehydrogenase |
| InterPro | IPR006096 | Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal |
| InterPro | IPR006097 | Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain |
| InterPro | IPR014362 | Glutamate dehydrogenase |
| InterPro | IPR033524 | Leu/Phe/Val dehydrogenases active site |
| InterPro | IPR033922 | NAD(P) binding domain of glutamate dehydrogenase |
| InterPro | IPR036291 | NAD(P)-binding domain superfamily |
| PFam | PF00208 | Glutamate/phenylalanine/leucine/valine dehydrogenase, C-terminal |
| PFam | PF02812 | Glutamate/phenylalanine/leucine/valine dehydrogenase, dimerisation domain |
| Superfamily | SSF51735 | NAD(P)-binding domain superfamily |
| Superfamily | SSF53223 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 31 | 12 | 30 |
| disruptive | 2 | 15 | 11 | 35 |
| missense | 2 | 13 | 9 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 65 | 11 | 25 |
| disruptive | 11 | 39 | 26 | 90 |
| missense | 9 | 29 | 23 | 62 |
PlasmoDB Total SNPs: 55
Non-coding: 35 | Synonymous: 14 | Nonsynonymous: 6 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9874251 | Glutamate dehydrogenase, the marker protein of Plasmodium falciparum--cloning,expression and characterization of the malarial enzyme. | Wagner JT, Ludemann H, ..., Krauth-Siegel RL | 10.1046/j.1432-1327.1998.2580813.x |
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 21756354 | Plasmodium falciparum glutamate dehydrogenase a is dispensable and not a drugtarget during erythrocytic development. | Storm J, Perner J, ..., Muller S | 10.1186/1475-2875-10-193 |
| 22342964 | Biochemical and structural characterization of Plasmodium falciparum glutamatedehydrogenase 2. | Zocher K, Fritz-Wolf K, ..., Becker K | 10.1016/j.molbiopara.2012.01.007 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31199842 | Plasmodium falciparum glutamate dehydrogenase is genetically conserved acrosseight malaria endemic states of India: Exploring new avenues of malariaelimination. | Ahmad A, Verma AK, ..., Bharti PK | 10.1371/journal.pone.0218210 |
| 34956931 | Merozoite Proteins Discovered by qRT-PCR-Based Transcriptome Screening ofPlasmodium falciparum. | Hou N, Li S, ..., Chen Q | https://pubmed.ncbi.nlm.nih.gov/34956931/ |
| 35128633 | Engineered yeast for efficient de novo synthesis of 7-dehydrocholesterol | Qu L, Xiu X, ..., Liu L | 10.1002/bit.28055 |
| 38040111 | Engineering and evolution of the complete Reductive Glycine Pathway inSaccharomyces cerevisiae for formate and CO(2) assimilation | Bysani VR, Alam AS, Bar-Even A, Machens F | 10.1016/j.ymben.2023.11.007 |
| 35942513 | Glutamine transport as a possible regulator of nitrogen catabolite repression inSaccharomyces cerevisiae | Georis I, Fayyad-Kazan M, ..., Dubois E | 10.1002/yea.3809 |