PF3D7_1416800 (KRS2)

Genome location: Pf3D7_14_v3:682,466..685,191(+)

Genome classification: Core

Function and Localization

Product Description: lysine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 6.1.1.6 (Lysine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005829	cytosol
Function	GO:0004824	lysine-tRNA ligase activity
Function	GO:0000049	tRNA binding
Process	GO:0006430	lysyl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.06	0.97
Trophozoite	expressed	0.36	0.78
Schizont	expressed	0.06	0.96
Gametocyte	expressed	0.54	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1416800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.12 | MFS: -2.912 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1025900)

Relative Growth Rate: 0.22 ± 0.23
Confidence: 4.31

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8ILS8

"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.20) with 6NRZ (Global RMSD=1.70)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.6	lysine-tRNA ligase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_170326

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	35	24	56
disruptive	9	60	28	103
missense	9	59	27	98

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	13	84	34	52
disruptive	29	127	64	187
missense	21	114	57	152

PlasmoDB Total SNPs: 96

Non-coding: 67 | Synonymous: 16 | Nonsynonymous: 13 | Stop Codon: 0

Protein Information

Protein Length: 674 | Molecular Weight (kDa): 79.164

UniProt ID(s): Q8ILS8

PDB ID(s): None

Isoelectric Point: 9.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002313	Lysine-tRNA ligase, class II
InterPro	IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)
InterPro	IPR012340	Nucleic acid-binding, OB-fold
InterPro	IPR018149	Lysyl-tRNA synthetase, class II, C-terminal
PFam	PF00152	Aminoacyl-tRNA synthetase, class II (D/K/N)
Superfamily	SSF50249	Nucleic acid-binding, OB-fold
Superfamily	SSF55681

PMID	Title	Authors	DOI/Link
12368864	Genome sequence of the human malaria parasite Plasmodium falciparum.	Gardner MJ, Hall N, ..., Barrell B	10.1038/nature01097
23444099	Selective inhibition of an apicoplastic aminoacyl-tRNA synthetase from Plasmodiumfalciparum.	Hoen R, Novoa EM, ..., Royo M	10.1002/cbic.201200620
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895