About Gene List

PF3D7_1417200 (NOT1)

Genome location: Pf3D7_14_v3:693,329..708,432(+)

Genome classification: Core

Function and Localization

Product Description: CCR4-NOT transcription complex subunit NOT1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0030014 CCR4-NOT complex
Component GO:0030015 CCR4-NOT core complex
Component GO:0000932 P-body
Component GO:0005829 cytosol
Component GO:0005634 nucleus
Function GO:0003729 mRNA binding
Function GO:0060090 molecular adaptor activity
Process GO:0017148 negative regulation of translation
Process GO:0000288 nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring expressed 2.60 0.14
Trophozoite expressed 1.99 0.18
Schizont expressed 1.88 0.21
Gametocyte not expressed 1.80 0.23

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_1417200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 15 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.061 | #Insertions: 12

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PY17X_1027900)

Modification: Tagged | RMgm-5095

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532996

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 47 228 123 268
disruptive 165 713 380 846
missense 86 556 315 660

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 96 446 168 314
disruptive 341 1511 668 1467
missense 174 1130 522 841

PlasmoDB Total SNPs: 595

Non-coding: 117 | Synonymous: 275 | Nonsynonymous: 203 | Stop Codon: 0

Protein Information

Protein Length: 4466 | Molecular Weight (kDa): 519.826

UniProt ID(s): Q8ILS4

PDB ID(s): None

Isoelectric Point: 7.25

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007196 CCR4-Not complex component, Not1, C-terminal
InterPro IPR024557 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
InterPro IPR040398 CCR4-NOT transcription complex subunit 1
PFam PF04054 CCR4-Not complex component, Not1, C-terminal
PFam PF12842 CCR4-Not complex, Not1 subunit, domain of unknown function DUF3819
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
12368864 Genome sequence of the human malaria parasite Plasmodium falciparum. Gardner MJ, Hall N, ..., Barrell B 10.1038/nature01097
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
27381095 The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. Bunnik EM, Batugedara G, ..., Le Roch KG 10.1186/s13059-016-1014-0
34673764 The Plasmodium NOT1-G paralogue is an essential regulator of sexual stagematuration and parasite transmission. Hart KJ, Power BJ, ..., Lindner SE 10.1371/journal.pbio.3001434
38352447 Global Release of Translational Repression Across Plasmodium's Host-to-VectorTransmission Event Rios KT, McGee JP, ..., Lindner SE 10.1101/2024.02.01.577866
38148574 The Plasmodium falciparum CCCH zinc finger protein MD3 regulates malegametocytogenesis through its interaction with RNA-binding proteins Farrukh A, Musabyimana JP, ..., Ngwa CJ 10.1111/mmi.15215