Product Description: H/ACA ribonucleoprotein complex subunit 4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 5.4.99.- (Transferring other groups.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031429 | box H/ACA snoRNP complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0009982 | pseudouridine synthase activity |
| Process | GO:0000495 | box H/ACA RNA 3'-end processing |
| Process | GO:1990481 | mRNA pseudouridine synthesis |
| Process | GO:0031118 | rRNA pseudouridine synthesis |
| Process | GO:0031120 | snRNA pseudouridine synthesis |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.63 | 0.70 |
| Trophozoite | expressed | 0.44 | 0.74 |
| Schizont | expressed | 0.09 | 0.94 |
| Gametocyte | expressed | 0.43 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1417500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.278 | MFS: -2.691 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1025200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILS0
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=2.07) with 2HVY (Global RMSD=2.65)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 5.4.99.- | Ex: tRNA pseudouridine38-40 synthase | SDSGTPGDPNEMEDTAtRNAPCMBFeCl2CuSO3HgCl2CuSO4AgNO3... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100327
Most Similar Human Ortholog: O60832
TM-align score: 0.85 | RMSD: 2.31
Seq Identity: 0.59 | Length: 413 / 466
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000130826 | dyskerin pseudouridine synthase 1 |
| ENSG00000165832 | TruB pseudouridine synthase family member 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 20 | 15 | 29 |
| disruptive | 4 | 23 | 17 | 35 |
| missense | 4 | 23 | 11 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 61 | 15 | 33 |
| disruptive | 11 | 55 | 36 | 89 |
| missense | 7 | 44 | 31 | 68 |
PlasmoDB Total SNPs: 118
Non-coding: 108 | Synonymous: 7 | Nonsynonymous: 3 | Stop Codon: 0
Protein Length: 466 | Molecular Weight (kDa): 52.942
UniProt ID(s): Q8ILS0
PDB ID(s): None
Isoelectric Point: 9.25
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002478 | PUA domain |
| InterPro | IPR002501 | Pseudouridine synthase II, N-terminal |
| InterPro | IPR004802 | tRNA pseudouridine synthase B family |
| InterPro | IPR012960 | Dyskerin-like |
| InterPro | IPR015947 | PUA-like superfamily |
| InterPro | IPR020103 | Pseudouridine synthase, catalytic domain superfamily |
| InterPro | IPR032819 | tRNA pseudouridylate synthase B, C-terminal |
| PFam | PF01472 | PUA domain |
| PFam | PF01509 | Pseudouridine synthase II, N-terminal |
| PFam | PF08068 | Dyskerin-like |
| PFam | PF16198 | tRNA pseudouridylate synthase B, C-terminal |
| Superfamily | SSF55120 | Pseudouridine synthase, catalytic domain superfamily |
| Superfamily | SSF88697 | PUA-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 31311819 | Base-pairing interactions between substrate RNA and H/ACA guide RNA modulate thekinetics of pseudouridylation, but not the affinity of substrate binding by H/ACAsmall nucleolar ribonucleoproteins | Kelly EK, Czekay DP, Kothe U | 10.1261/rna.071043.119 |
| 33823543 | Eukaryote specific RNA and protein features facilitate assembly and catalysis ofH/ACA snoRNPs | Trucks S, Hanspach G, Hengesbach M | 10.1093/nar/gkab177 |
| 37818102 | Human SHQ1 variants R335C and A426V lead to severe ribosome biogenesis defectswhen expressed in yeast | Alidou-D'Anjou I, Patel A, Sleiman S, Dragon F | 10.3389/fgene.2023.1240416 |