Product Description: DNA replication licensing factor MCM2
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0042555 | MCM complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0003688 | DNA replication origin binding |
| Function | GO:0003723 | RNA binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Process | GO:0006260 | DNA replication |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0000727 | double-strand break repair via break-induced replication |
| Process | GO:1902975 | mitotic DNA replication initiation |
| Process | GO:0009657 | plastid organization |
| Process | GO:0006267 | pre-replicative complex assembly involved in nuclear cell cycle DNA replication |
| Process | GO:0051726 | regulation of cell cycle |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.13 | 0.94 |
| Trophozoite | expressed | 1.05 | 0.48 |
| Schizont | expressed | 0.57 | 0.69 |
| Gametocyte | expressed | 0.70 | 0.63 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1417800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.147 | MFS: -2.829 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1024900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8ILR7
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.12) with 4R7Z (Global RMSD=6.90)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101336
Most Similar Human Ortholog: P49736
TM-align score: 0.79 | RMSD: 3.88
Seq Identity: 0.37 | Length: 789 / 971
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000073111 | minichromosome maintenance complex component 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 54 | 36 | 65 |
| disruptive | 5 | 9 | 7 | 47 |
| missense | 5 | 9 | 7 | 37 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 151 | 34 | 40 |
| disruptive | 10 | 27 | 31 | 69 |
| missense | 8 | 21 | 20 | 55 |
PlasmoDB Total SNPs: 107
Non-coding: 72 | Synonymous: 25 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 971 | Molecular Weight (kDa): 111.496
PDB ID(s): None
Isoelectric Point: 5.44
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001208 | MCM domain |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR008045 | DNA replication licensing factor Mcm2 |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR027925 | MCM N-terminal domain |
| InterPro | IPR031327 | Mini-chromosome maintenance protein |
| InterPro | IPR033762 | MCM OB domain |
| InterPro | IPR041562 | MCM, AAA-lid domain |
| PFam | PF00493 | MCM domain |
| PFam | PF14551 | MCM N-terminal domain |
| PFam | PF17207 | MCM OB domain |
| PFam | PF17855 | MCM, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 16257456 | Pre-replication complex organization in the atypical DNA replication cycle ofPlasmodium falciparum: characterization of the mini-chromosome maintenance (MCM)complex formation. | Patterson S, Robert C, ..., Chakrabarti D | 10.1016/j.molbiopara.2005.09.006 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30726238 | A mutagenesis screen for essential plastid biogenesis genes in human malariaparasites. | Tang Y, Meister TR, ..., Yeh E | 10.1371/journal.pbio.3000136 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |