Product Description: ATP-dependent RNA helicase DBP4, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Function | GO:0003724 | RNA helicase activity |
| Process | GO:0006364 | rRNA processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 1.19 | 0.49 |
| Trophozoite | expressed | 0.51 | 0.72 |
| Schizont | expressed | 0.17 | 0.91 |
| Gametocyte | expressed | 0.47 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_1418900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.83 | #Insertions: 2
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1024300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C6S3H8
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.09) with 6L5N (Global RMSD=2.82)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102138
Most Similar Human Ortholog: A0A3B3ISR7
TM-align score: 0.72 | RMSD: 3.38
Seq Identity: 0.33 | Length: 530 / 876
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000178105 | DEAD-box helicase 10 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 55 | 30 | 53 |
| disruptive | 22 | 81 | 59 | 144 |
| missense | 15 | 73 | 52 | 129 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 20 | 96 | 43 | 64 |
| disruptive | 39 | 206 | 86 | 230 |
| missense | 21 | 168 | 78 | 183 |
PlasmoDB Total SNPs: 160
Non-coding: 81 | Synonymous: 60 | Nonsynonymous: 19 | Stop Codon: 0
Protein Length: 876 | Molecular Weight (kDa): 103.746
UniProt ID(s): A0A346QRU7, A0A346QRU8, A7U5X3, C6S3H8
PDB ID(s): None
Isoelectric Point: 9.09
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR025313 | Domain of unknown function DUF4217 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368864 | Genome sequence of the human malaria parasite Plasmodium falciparum. | Gardner MJ, Hall N, ..., Barrell B | 10.1038/nature01097 |
| 9199348 | The rRNA-processing function of the yeast U14 small nucleolar RNA can be rescuedby a conserved RNA helicase-like protein | Liang WQ, Clark JA, Fournier MJ | 10.1128/MCB.17.7.4124 |
| 35650250 | Artificial intelligence-assisted cryoEM structure of Bfr2-Lcp5 complex observedin the yeast small subunit processome | Zhao Y, Rai J, ..., Li H | 10.1038/s42003-022-03500-y |